The region in myosin S-1 that may be involved in energy transduction.
The region in myosin S-1 that may be involved in energy transduction.
复制标题
肌球蛋白 S-1 中可能参与能量转导的区域。
DOI：
10.1007/978-1-4615-2872-2_22
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发表时间：
1993
影响因子：
--
通讯作者：
Bivin,DB
中科院分区：
文献类型：
--
作者：
Morales,MF;Ue,K;Bivin,DB
新报道的关于结合核苷酸上的点与肌球蛋白S-1重链上的点之间某些邻近点的结构信息被纳入先前报道的[Botts， J. Thomason， J.F. & Morales， M.F.Proc]。Nat. Acad. Sci。[j] .中国有色金属学报，2009,22(3):444 - 444。由此产生的增强结构随后用于识别某些功能（例如，atp可扰动色氨酸），并解释某些观察结果（例如，一些关于结合的Mg2+在tnbs标记的Lys-83的光谱响应中的作用，以及一些关于S-1 CD信号对核苷酸结合和温度变化的响应）。这些考虑导致了这样的建议：一条50 kDa“结构域”链（残基510 - 540）和一条20 kDa“结构域”链（残基697-719）参与将核苷酸结合和水解的作用传递到环（由相同的“结构域”组成），从而到达主要的(S-l)-肌动蛋白界面。
Newly-reported structural information about certain proximities between points on bound nucleotide and points on the heavy chain of myosin S-1 are incorporated into a previously-reported [Botts, J. Thomason, J.F. & Morales, M.F.Proc. Nat. Acad. Sci. USA,86, 2204-2208 (1989)] structure of S-l. The resulting, enhanced structure is then used to identify some functionalities (e.g., the ATP-perturbable tryptophans), and to explain certain observations (e.g., some concerning the role of bound Mg2+in the spectral response of TNBS-labelled Lys-83, and some concerning the response of the S-1 CD signal to nucleotide binding and to temperature change). These considerations lead to the suggestion that a strand of the 50 kDa “domain” (residues 510 to 540), and a strand of the 20 kDa ‘domain” (residues 697–719) are involved in transmitting the effects of nucleotide binding and hydrolysis to the loop (constituted from the same “domains”) that reaches a major (S-l)-actin interface.