Seasonal Influenza Vaccine Provides Priming For A/H1N1 Immunization
Seasonal Influenza Vaccine Provides Priming For A/H1N1 Immunization
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DOI：
10.1126/scitranslmed.3000564
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发表时间：
2009-12-23
影响因子：
17.1
通讯作者：
Rappuoli, Rino
中科院分区：
文献类型：
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作者：
Del Giudice, Giuseppe;Stittelaar, Koert J.;Rappuoli, Rino
针对 2009 年甲型 H1N1 流感病毒的大规模疫苗接种正在全球范围内实施，疫苗配方各异。由于疫苗制备的紧迫性，关于影响疫苗有效性的变量的信息很少。具体而言，尚不清楚先前感染季节性流感或接种季节性流感疫苗是否会影响对疫苗的反应。同样，疫苗佐剂在疫苗反应中的作用尚不清楚。为了解决这些问题，我们给雪貂注射了季节性流感疫苗，然后接种了含有或不含水包油佐剂 MF59 的 2009 A/H1N1 疫苗。结果表明，季节性流感疫苗虽然没有诱导针对 2009 A/H1N1 病毒的功能性抗体，但在注射一剂 2009 A/H1N1 疫苗后提供了免疫启动作用，并允许产生针对 2009 A/H1N1 病毒的保护性抗体。不含佐剂的疫苗显着降低了肺部的病毒载量，但不能防止感染。只有含有佐剂的疫苗才能完全预防肺部和鼻腔感染。
Mass vaccination against the 2009 A/H1N1 influenza virus, with different vaccine formulations, is being implemented globally. Because of the urgency with which the vaccine has been prepared, little information has been gathered on variables that influence the effectiveness of the vaccine. Specifically, it is not clear whether priming by previous infection with or vaccination against seasonal influenza affects the response to the vaccine. Similarly, the role of vaccine adjuvants in vaccine response is not known. To address these questions, we injected ferrets with seasonal influenza vaccine and then vaccinated with 2009 A/H1N1 vaccine with or without the oil-in-water adjuvant MF59. The results show that the seasonal flu vaccine, although it did not induce functional antibodies against the 2009 A/H1N1 virus, provided immunological priming and allowed production of protective antibodies to 2009 A/H1N1 after one dose of 2009 A/H1N1 vaccine. The vaccine given without adjuvant significantly reduced viral load in the lungs but did not protect from infection. Only the vaccine with adjuvant completely prevented both pulmonary and nasal infection.