SV40-TRANSFORMED SIMIAN CELLS SUPPORT THE REPLICATION OF EARLY SV40 MUTANTS
SV40-TRANSFORMED SIMIAN CELLS SUPPORT THE REPLICATION OF EARLY SV40 MUTANTS
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DOI：
10.1016/0092-8674(81)90282-8
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发表时间：
1981-01-01
期刊：
影响因子：
64.5
通讯作者：
GLUZMAN, Y
中科院分区：
文献类型：
--
作者：
GLUZMAN, Y
CV-1是一种已建立的允许SV 40裂解生长的非洲绿色猴肾细胞系，用编码野生型T抗原的SV 40的起源缺陷突变体转化CV-1。建立了三个转化株系（COS-1，-3，-7）;它们含有T抗原，保留了对SV 40裂解生长的完全容许性，支持tsA 209病毒在40 ℃复制。C，并支持复制的纯群体的SV 40突变体与删除在早期区域。COS-1系含有SV 40 DNA完整早期区的单个整合拷贝。这些细胞可能是重组SV 40病毒纯群体繁殖的宿主。
CV-1, an established line of African green monkey kidney cells permissive for lytic growth of SV40, were transformed by an origin-defective mutant of SV40 which codes for wild-type T antigen. Three transformed lines (COS-1, -3, -7) were established; they contained T antigen, retained complete permissiveness for lytic growth of SV40, supported the replication of tsA209 virus at 40.degree. C, and supported the replication of pure populations of SV40 mutants with deletions in the early region. Line COS-1 contains a single integrated copy of the complete early region of SV40 DNA. The cells are possible hosts for the propagation of pure populations of recombinant SV40 viruses.