[Effects of microbial agent inoculation on bacterial community diversity in the process of pig manure composting].
[Effects of microbial agent inoculation on bacterial community diversity in the process of pig manure composting].
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发表时间：
2009-08
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通讯作者：
Kaizhi Xie;Pei-zhi Xu;Fabao Zhang;Jiansheng Chen;Shuanghu Tang;Xu Huang;Chaozhan Yan;Wenjie Gu
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作者：
Kaizhi Xie;Pei-zhi Xu;Fabao Zhang;Jiansheng Chen;Shuanghu Tang;Xu Huang;Chaozhan Yan;Wenjie Gu
采用PCR-DGGE方法研究了外源微生物菌剂对鲜猪粪高温好氧堆肥过程中细菌群落多样性的影响。接种外源微生物菌剂促进了堆肥化进程，高温期比未接种的提前2 d。DGGE图谱分析表明，堆肥过程中优势菌发生了明显变化，不同堆肥时期细菌群落的Shannon-Wiener指数变化明显。DGGE区分带测序结果表明，粪梭菌粪亚种（Clostridium stercorarium subsp.）在整个堆肥过程中，嗜热菌是优势菌群。未培养的凝结芽孢杆菌和热纤梭菌在接种后第10和16天成为主要菌群，而未培养的厚壁菌门和δ变形杆菌分别在未接种处理的第5和16天成为优势菌群。未培养的土壤细菌主要出现在堆肥腐熟初期和高温期，而未培养的嗜热球脲杆菌（Ureibacillusthermosphaericussp.）UPGMC聚类分析表明，外源微生物菌剂接种对堆肥不同时期的细菌群落结构有明显影响，堆肥过程DGGE图谱的主成分分析表明，细菌群落主要受外源微生物菌剂接种的影响。
PCR-DGGE method was adopted to study the effects of inoculating exogenous microbial agent on the bacterial community diversity in the process of fresh pig manure high-temperature aerobic composting. Exogenous microbial agent inoculation promoted the composting process, with the high-temperature period being advanced by 2 days than that of non-inoculation. DGGE pattern analysis showed that during composting, the dominant bacteria changed significantly, with the Shannon-Wiener index of bacterial community at different composting periods varied obviously. The sequencing of DGGE-distinguished bands showed that Clostridium stercorarium subsp. thermolacticum sp. was the dominant group in the whole composting process. Uncultured bacteria Bacillus coagulans sp. and Clostridium thermocellum sp. became the main groups on the 10th and 16th day after microbial agent inoculation, while uncultured Firmicutes sp. and delta proteobacterium became the dominant groups on the 5th and 16th days in the treatment non-inoculation, respectively. Un-dominant group Ureibacillus thermosphaericu sp. and uncultured Silvimonas sp. appeared in the late period of well rotted composting, while uncultured soil bacteria mainly appeared in the initial and high-temperature periods. UPGMC cluster analysis showed that exogenous microbial agent inoculation obviously affected the bacterial community structure in different composting periods, and the main component analysis of DGGE patterns in composting process showed that the bacterial community was mainly affected by the exogenous microbial agent inoculation.