Definition of dendritic cell subpopulations present in the spleen, Peyer's patches, lymph nodes, and skin of the mouse
Definition of dendritic cell subpopulations present in the spleen, Peyer's patches, lymph nodes, and skin of the mouse
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DOI：
10.1182/blood.v93.2.590.402k11_590_598
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发表时间：
1999-01-15
期刊：
影响因子：
20.3
通讯作者：
Ardavin, C
中科院分区：
文献类型：
--
作者：
Anjuère, F;Martín, P;Ardavin, C
树突状细胞（DC）是一种高效的抗原呈递细胞（APC），在产生针对微生物病原体和肿瘤的免疫应答中具有重要功能。虽然在过去的几年中，我们对DC生物学的理解已经显着增加，不同的DC亚群的精确表征仍然有待实现关于它们的表型和谱系关系。在这份报告中，我们已经广泛研究了DC亚群存在于胸腺，脾脏，派尔氏干，淋巴结（LN）和皮肤的小鼠。胸腺DC和60%脾DC具有淋巴样DC表型，即，CD 8（+）DEC-205（高）Mac-1（低），而40%脾DC具有髓样DC表型，即，CD 8（-）DEC-205（低）Mac-1（高）。CD 8（+）和CD 8- DC都是白细胞功能相关抗原-1（LFA-1）（高）和高粘附性。在派伊尔集合淋巴结内，大多数DC对应于CD 8（+）DEC-205（高）Mac-1（低）淋巴类别。在LN中，与CD 8+和CD 8- DC一起，描述了具有淋巴样DC特征的另外的非粘附CD 8（int）LFA-1（int）亚群。最后，在皮肤中，表皮朗格汉斯细胞（LC）和真皮DC都是CD 8-DEC-205（高）Mac-1（高），并且不表达LFA-1。有趣的是，LC迁移实验表明LC在迁移到LN时经历了CD 8和LFA-1的上调，支持LC属于CD 8（+）淋巴谱系的假设。(C)1999年，美国血液学会。
Dendritic cells (DC) are highly efficient antigen-presenting cells (APC) that have an essential function in the development of immune responses against microbial pathogens and tumors. Although during the past few years our understanding of DC biology has remarkably increased, a precise characterization of the different DC subpopulations remains to be achieved with regard to their phenotype and lineage relationships. In this report, we have extensively studied the DC subpopulations present in the thymus, spleen, Peyer's parches, lymph nodes (LN) and skin of the mouse. Thymus DC and 60% spleen DC have a lymphoid DC phenotype, ie, CD8(+) DEC-205(high) Mac-1(low), whereas 40% spleen DC have a myeloid DC phenotype, ie, CD8(-) DEC-205(low) Mac-1(high). Both CD8(+) and CD8- DC are leukocyte function-associated antigen-1 (LFA-1)(high) and highly adherent. Within Peyer's patches the majority of DC correspond to the CD8(+) DEC-205(high) Mac-1(low) lymphoid category. In the LN, together with CD8+ and CD8- DC, an additional nonadherent CD8(int) LFA-1(int) subpopulation with lymphoid DC characteristics is described. Finally, in the skin both epidermal Langerhans cells (LC) and dermal DC are CD8-DEC-205(high) Mac-1(high) , and do not express LFA-1. Interestingly, LC migration experiments indicate that LC underwent the upregulation of CD8 and LFA-1 upon migration to the LN, supporting the hypothesis that LC belong to the CD8(+) lymphoid lineage. (C) 1999 by The American Society of Hematology.