STRUCTURE AND MORPHOGENESIS OF DUGBE VIRUS (BUNYAVIRIDAE, NAIROVIRUS) STUDIED BY IMMUNOGOLD ELECTRON-MICROSCOPY OF ULTRATHIN CRYOSECTIONS
STRUCTURE AND MORPHOGENESIS OF DUGBE VIRUS (BUNYAVIRIDAE, NAIROVIRUS) STUDIED BY IMMUNOGOLD ELECTRON-MICROSCOPY OF ULTRATHIN CRYOSECTIONS
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DOI：
10.1016/0168-1702(91)90033-r
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发表时间：
1991-11-01
期刊：
影响因子：
5
通讯作者：
NUTTALL, PA
中科院分区：
文献类型：
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作者：
BOOTH, TF;GOULD, EA;NUTTALL, PA
我们使用免疫金电子显微镜研究了培养的猪肾 (PS) 细胞和蜱细胞系 (Ra 243) 中杜格贝 (DUG) 病毒（布尼亚病毒科、内罗病毒）的结构和形态发生。 DUG 病毒是一种蜱传虫媒病毒，被认为对健康危害较低，与克里米亚刚果出血热 (CCHF) 病毒具有抗原性和遗传相关性（Marriott 等，1990）。我们使用单克隆抗体对超薄冷冻切片进行免疫金标记和免疫荧光技术，研究了 DUG 病毒颗粒的成熟和细胞内转运，作为其他更具致病性的内罗病毒的模型。球形 DUG 病毒颗粒直径约为 90 nm，有 5 nm 厚的膜，上面覆盖着 5-7 nm 长的突出物或“尖刺”。这些突起形成空心圆柱形形态单元，直径约 5 nm。 DUG 病毒感染仅在哺乳动物细胞中引起轻微的细胞病变作用，而在蜱细胞中则没有。 DUG 病毒颗粒由高尔基复合体出芽组装而成，其中 DUG 病毒糖蛋白 G1 积聚在源自高尔基池的囊泡中。核衣壳蛋白 N 在整个细胞质的分散灶中积累，这似乎与 DUG 病毒颗粒的有限成熟有关。在蜱细胞中观察到的出芽病毒颗粒数量的减少与观察到的细胞病理学的减少相关。
We have studied the structure and morphogenesis of Dugbe (DUG) virus (Bunyaviridae, Nairovirus) in cultured porcine kidney (PS) cells and a tick cell line (Ra 243) using immunogold electron microscopy. DUG virus is a tickborne arbovirus, considered to be a low health hazard, that is antigenically and genetically related to Crimean Congo haemorrhagic fever (CCHF) virus (Marriott et al., 1990). We have investigated the maturation and intracellular transport of DUG virus particles as a model for other more pathogenic nairoviruses using monoclonal antibodies for immunogold labelling of ultrathin cryosections and immunofluorescence techniques. The spherical DUG virus particle measures about 90 nm in diameter, with a 5 nm thick membrane covered by 5-7 nm long projections or "spikes". These projections form hollow cylindrical morphological units, about 5 nm in diameter. DUG virus infection caused only a slight cytopathogenic effect in mammalian cells and none in tick cells. DUG virus particles assembled by budding from the Golgi complex, where the DUG virus glycoprotein G1 accumulated in vesicles originating from Golgi cisternae. The nucleocapsid protein N accumulated in scattered foci throughout the cytoplasm, and this appears to be related to the limited maturation of DUG virus particles that occurred. The reduced number of budding virus particles observed in tick cells was correlated with the reduced cytopathology observed.