GENETIC-ANALYSIS OF THE RECG LOCUS OF ESCHERICHIA-COLI-K-12 AND OF ITS ROLE IN RECOMBINATION AND DNA-REPAIR
GENETIC-ANALYSIS OF THE RECG LOCUS OF ESCHERICHIA-COLI-K-12 AND OF ITS ROLE IN RECOMBINATION AND DNA-REPAIR
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DOI：
10.1128/jb.173.3.1004-1011.1991
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发表时间：
1991-02-01
影响因子：
3.2
通讯作者：
BUCKMAN, C
中科院分区：
文献类型：
--
作者：
LLOYD, RG;BUCKMAN, C
我们描述了一种在大肠杆菌中降低同源重组和DNA修复效率的转座子插入。在当前的连锁图谱上，插入片段rec-258位于PYRE和DGO之间。根据与PYRE的连锁和与所克隆的rec+基因的互补研究，rec-258被鉴定为Storm等人首次报道的recG基因座的一个等位基因。(P.K.Storm，W.P.M.Hoekstra，P.G.de Haan，C.Verhoef，Mutat.书13：9-17,1971)。在野生型、recB recc SBCA和recB recc sbcB SBCC遗传背景中，recG258突变赋予人对丝裂霉素C和紫外线的敏感性，并使接合重组缺陷3-10倍。它似乎不影响野生型中的质粒重组。RecG258单个突变体对电离辐射也很敏感。SOS反应是正常诱导的，尽管基础表达水平提高了两到三倍。进一步的遗传研究表明，在每种情况下，recB和recG和recG recJ双突变株对紫外光的敏感性都比各自的单个突变株高得多。然而，未发现recG258与recf、recN或recQ突变之间的协同作用。结论是，recG不属于任何被接受的影响重组和DNA修复的基因组。
We describe a transposon insertion that reduces the efficiency of homologous recombination and DNA repair in Escherichia coli. The insertion, rec-258, was located between pyrE and dgo at min 82.1 on the current linkage map. On the basis of linkage to pyrE and complementation studies with the cloned rec+ gene, rec-258 was identified as an allele of the recG locus first reported by Storm et al. (P. K. Storm, W. P. M. Hoekstra, P. G. De Haan, and C. Verhoef, Mutat. Res. 13:9-17, 1971). The recG258 mutation confers sensitivity to mitomycin C and UV light and a 3- to 10-fold deficiency in conjugational recombination in wild-type, recB recC sbcA, and recB recC sbcB sbcC genetic backgrounds. It does not appear to affect plasmid recombination in the wild-type. A recG258 single mutant is also sensitive to ionizing radiation. The SOS response is induced normally, although the basal level of expression is elevated two- to threefold. Further genetic studies revealed that recB and recG and recG recJ double mutants are much more sensitive to UV light than the respective single mutants in each case. However, no synergistic interactions were discovered between recG258 and mutations in recF, recN, or recQ. It is concluded that recG does not fall within any of the accepted groups of genes that affect recombination and DNA repair.