Transcription profiling and regulation of fat metabolism genes in diapausing adults of the mosquito Culex pipiens
Transcription profiling and regulation of fat metabolism genes in diapausing adults of the mosquito Culex pipiens
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DOI：
10.1152/physiolgenomics.00095.2009
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发表时间：
2009-11-06
影响因子：
4.6
通讯作者：
Denlinger, David L.
中科院分区：
文献类型：
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作者：
Sim, Cheolho;Denlinger, David L.
SimC，Denlinger DL.尖音库蚊滞育成虫脂肪代谢基因的转录谱及其调控。Physiol Genomics 39：202-209，2009.首次发表于2009年8月25日; doi：10.1152/physiolgenomics.00095.2009.-在北美传播西尼罗河病毒的库蚊（Culex pipiens）以成虫滞育（休眠）方式越冬，这是由秋季短日照和低温造成的。作为对这些环境信号的回应，雌性停止以血液为食，而是寻找花蜜来源，以产生冬季生存所需的大量脂质储备。为了确定调控网络，调节脂肪的积累和脂肪消耗滞育期间，我们比较了脂肪相关基因的表达，从nondiapausing女性的表达，这些相同的基因在早期和晚期滞育和滞育终止。在我们检测的31个基因中，有4个基因在滞育早期比在非滞育中表达更高，而14个基因在滞育早期表达下调。在滞育早期向滞育晚期过渡的过程中，与脂肪代谢相关的19个基因表达上调。如前所述，在滞育早期，脂肪酸合成酶fas-1表达上调。许多脂肪代谢基因，包括多个动力学类和基因参与β-氧化，能量生成步骤，被抑制在早期滞育，但在滞育后期和滞育终止高度表达。RNA干扰（RNAi）分析表明，fas-1基因和其它基因（fas-3和fabp）在滞育早期的脂肪储存中起重要作用。当这些基因的表达受到抑制时，雌性蚊子就无法隔离越冬所需的脂质。
Sim C, Denlinger DL. Transcription profiling and regulation of fat metabolism genes in diapausing adults of the mosquito Culex pipiens. Physiol Genomics 39: 202-209, 2009. First published August 25, 2009; doi: 10.1152/physiolgenomics.00095.2009.-Culex pipiens, the mosquito that vectors West Nile virus in North America, overwinters in an adult diapause (dormancy) that is programmed by the short day length and low temperatures of autumn. In response to these environmental signals, females cease feeding on blood and instead seek sources of nectar used to generate the huge lipid reserves required for winter survival. To identify regulatory networks that regulate fat accumulation and fat consumption during diapause, we compared expression of fat-related genes from nondiapausing females with expression of those same genes in early and late diapause and at diapause termination. Among the 31 genes we examined, 4 were expressed more highly in early diapause than in nondiapause, while 14 genes were downregulated in early diapause. In the transition from early to late diapause, 19 genes related to fat metabolism were upregulated. As reported previously, fatty acid synthase, identified as fas-1 in this study, was upregulated in early diapause. Numerous fat metabolism genes, including multiple kinetic classes and genes involved in beta-oxidation, an energy-generation step, were suppressed in early diapause but were highly expressed in late diapause and at diapause termination. RNA interference (RNAi) analysis revealed that the fas-1 gene and others (fas-3 and fabp) have important roles in fat storage during early diapause. When expression of these genes is suppressed, female mosquitoes fail to sequester the lipids needed for overwintering.