Cannabidiolic-acid synthase, the chemotype-determining enzyme in the fiber-type Cannabis sativa
Cannabidiolic-acid synthase, the chemotype-determining enzyme in the fiber-type Cannabis sativa
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DOI：
10.1016/j.febslet.2007.05.043
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发表时间：
2007-06-26
期刊：
影响因子：
3.5
通讯作者：
Morimoto, Satoshi
中科院分区：
文献类型：
--
作者：
Taura, Futoshi;Sirikantaramas, Supaart;Morimoto, Satoshi
大麻二醇酸（CBDA）合成酶是催化大麻二醇酸氧化环化成 CBDA 的酶，CBDA 是纤维型大麻的主要大麻素成分。我们使用退化引物和基因特异引物，通过反转录和聚合酶链反应克隆了编码 CBDA 合成酶的新型 cDNA。重组酶的生化特性表明，CBDA 合成酶是一种共价黄素化氧化酶。CBDA 合成酶的结构和功能特性与四氢大麻酚酸（THCA）合成酶十分相似，后者负责药物型大麻植物中主要大麻素 THCA 的生物合成。(c) 2007 欧洲生化协会联合会。由 Elsevier B.V 出版。保留所有权利。
Cannabidiolic-acid (CBDA) synthase is the enzyme that catalyzes oxidative cyclization of cannabigerolic-acid into CBDA, the dominant cannabinoid constituent of the fiber-type Cannabis sativa. We cloned a novel cDNA encoding CBDA synthase by reverse transcription and polymerase chain reactions with degenerate and gene-specific primers. Biochemical characterization of the recombinant enzyme demonstrated that CBDA synthase is a covalently flavinylated oxidase. The structural and functional properties of CBDA synthase are quite similar to those of tetrahydrocannabinolic-acid (THCA) synthase, which is responsible for the biosynthesis of THCA, the major cannabinoid in drug-type Cannabis plants. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.