Ano1 is a selective marker of interstitial cells of Cajal in the human and mouse gastrointestinal tract
Ano1 is a selective marker of interstitial cells of Cajal in the human and mouse gastrointestinal tract
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DOI：
10.1152/ajpgi.00074.2009
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发表时间：
2009-06-01
影响因子：
4.5
通讯作者：
Farrugia, Gianrico
中科院分区：
文献类型：
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作者：
Gomez-Pinilla, Pedro J.;Gibbons, Simon J.;Farrugia, Gianrico
Gomez-Pinilla PJ, Gibbons SJ, Bardsley MR, Lorincz A, Pozo MJ, Pasricha PJ, Van de Rijn M, West RB, Sarr MG, Kendrick ML, Cima RR, Dozois EJ, Larson DW, Ordog T, Farrugia G. Ano1 是人和小鼠胃肠道中 Cajal 间质细胞的选择性标记物。 Am J Physiol Gastrointest Liver Physiol 296：G1370-G1381，2009。首次发表于 2009 年 4 月 16 日； doi:10.1152/ajpgi.00074.2009.-Cajal 间质细胞 (ICC) 群体在多种胃肠道神经肌肉疾病中发生改变。 ICC 通常通过 Kit (CD117) 的超微结构和表达来识别，Kit (CD117) 是一种也在肥大细胞上表达的蛋白质。目前还没有其他分子标记可以独立识别 ICC。 ANO1（DOG1、TMEM16A）（一种 Ca2+ 激活的 Cl- 通道）在胃肠道间质瘤中的表达表明它可能可用作 ICC 标记。因此，本研究的目的是确定 Ano1 与 Kit 相比的免疫反应性分布，并确定 Ano1 是否是人类和小鼠 ICC 的可靠标记。使用单独的 Ano1 抗体和与 Kit 的抗体组合，通过免疫荧光标记研究了 Ano1 在人和小鼠胃、小肠和结肠中的表达。通过落射荧光和共聚焦显微镜证明了免疫反应性的共定位。在固有肌层中，Ano1 免疫反应性仅限于具有 ICC 形态和分布的细胞。固有肌层中的所有 Ano1 阳性细胞也均为 Kit 阳性。表达 Kit 的肥大细胞不是 Ano1 阳性。人体组织粘膜及粘膜下层部分非ICC呈Ano1阳性，而Kit阴性。人胃固有肌层中的少数 (3.2%) Ano1 阳性细胞被 Kit 弱标记。 Ano1 标记所有类别的 ICC，是研究小鼠和人体组织中 ICC 分布的高度特异性标记物，与 Kit 相比具有优势，因为它不标记肥大细胞。
Gomez-Pinilla PJ, Gibbons SJ, Bardsley MR, Lorincz A, Pozo MJ, Pasricha PJ, Van de Rijn M, West RB, Sarr MG, Kendrick ML, Cima RR, Dozois EJ, Larson DW, Ordog T, Farrugia G. Ano1 is a selective marker of interstitial cells of Cajal in the human and mouse gastrointestinal tract. Am J Physiol Gastrointest Liver Physiol 296: G1370-G1381, 2009. First published April 16, 2009; doi:10.1152/ajpgi.00074.2009.-Populations of interstitial cells of Cajal (ICC) are altered in several gastrointestinal neuromuscular disorders. ICC are identified typically by ultrastructure and expression of Kit (CD117), a protein that is also expressed on mast cells. No other molecular marker currently exists to independently identify ICC. The expression of ANO1 (DOG1, TMEM16A), a Ca2+-activated Cl- channel, in gastrointestinal stromal tumors suggests it may be useful as an ICC marker. The aims of this study were therefore to determine the distribution of Ano1 immunoreactivity compared with Kit and to establish whether Ano1 is a reliable marker for human and mouse ICC. Expression of Ano1 in human and mouse stomach, small intestine, and colon was investigated by immunofluorescence labeling using antibodies to Ano1 alone and in combination with antibodies to Kit. Colocalization of immunoreactivity was demonstrated by epifluorescence and confocal microscopy. In the muscularis propria, Ano1 immunoreactivity was restricted to cells with the morphology and distribution of ICC. All Ano1-positive cells in the muscularis propria were also Kit positive. Kit-expressing mast cells were not Ano1 positive. Some non-ICC in the mucosa and submucosa of human tissues were Ano1 positive but Kit negative. A few (3.2%) Ano1-positive cells in the human gastric muscularis propria were labeled weakly for Kit. Ano1 labels all classes of ICC and represents a highly specific marker for studying the distribution of ICC in mouse and human tissues with an advantage over Kit since it does not label mast cells.