Screening and identification of plasma lncRNAs uc.48+and NR_105053 as potential novel biomarkers for cured pulmonary tuberculosis
Screening and identification of plasma lncRNAs uc.48+and NR_105053 as potential novel biomarkers for cured pulmonary tuberculosis
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筛选和鉴定血浆lncRNA uc.48和NR_105053作为治愈肺结核的潜在新型生物标志物
DOI：
10.1016/j.ijid.2020.01.005
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发表时间：
2020-03-01
影响因子：
8.4
通讯作者：
Li, Ji-Cheng
中科院分区：
文献类型：
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作者：
Li, Zhi-Bin;Han, Yu-Shuai;Li, Ji-Cheng
背景：结核病（TB）治疗需要很长时间，并且缺乏确定TB治愈的金标准测试。这可能导致结核病患者提前出院，从而增加疾病传播和耐药性的风险。血浆lncRNA可能作为潜在的生物标志物，以评估结核病治愈在efficient和precise manning.Methods：lncRNA微阵列分析用于筛选差异表达的血浆lncRNA在未经治疗的结核病和治愈的TB受试者。通过qPCR验证lncRNA的表达水平。结果：肺结核患者肺组织中lncRNAs uc.48+（p < 0.001）和NR_105053（p = 0.03）的表达水平与肺结核治愈组相比差异有统计学意义。uc.48 +的预测靶基因为EP300、BAI1，NR_105053的预测靶基因为TLR9、MYD88、BAI1。结论：lncRNAs uc.48+和NR_105053可作为区分结核病初治者和治愈者的潜在生物标志物。本研究为评价结核病治疗效果提供了实验依据，也可能为结核病的病理机制提供新的线索。(C)2020作者（S）由爱思唯尔有限公司代表国际传染病学会出版。
Background: Tuberculosis (TB) treatment takes a long time, and a gold standard test to define TB cure is lacking. This may lead to early discharge of TB patients, resulting in an increased risk of disease transmission and drug resistance. Plasma lncRNAs might act as potential biomarkers to evaluate TB cure in an efficient and precise manner.Methods: A lncRNA microarray assay was used to screen differentially expressed plasma lncRNAs in untreated TB and cured TB subjects. The expression levels of lncRNAs were verified by qPCR. Target genes of lncRNAs were predicted using a coding-non-coding gene co-expression network and mRNA-lncRNA-miRNA interaction network analysis.Results: The expression levels of lncRNAs uc.48+ (p < 0.001) and NR_105053 (p = 0.03) were found to differ significantly between the untreated TB group and the cured TB group. The predicted target genes of uc.48 + were EP300, BAI1 and NR_105053 were TLR9, MYD88, BAI1, respectively. A predictive model for cured TB was established by the combination of uc.48+ and NR_105053 expression, with a sensitivity of 90.00% and specificity of 86.36%, and an area under the curve (AUC) value of 0.945.Conclusions: lncRNAs uc.48+ and NR_105053 may serve as potential biomarkers to distinguish between untreated TB patients and cured TB subjects. This study provides an experimental basis to evaluate the effect of TB treatment and may also provide new clues to the pathological mechanisms of TB. (C) 2020 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.