Characterisation of peripheral blood mononuclear cell microRNA in hepatitis B-related acute-on-chronic liver failure.
Characterisation of peripheral blood mononuclear cell microRNA in hepatitis B-related acute-on-chronic liver failure.
复制标题
乙型肝炎相关慢加急性肝衰竭中外周血单核细胞 microRNA 的特征
DOI：
10.1038/srep13098
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发表时间：
2015-08-12
影响因子：
4.6
通讯作者：
Li J
中科院分区：
文献类型：
--
作者：
Ding W;Xin J;Jiang L;Zhou Q;Wu T;Shi D;Lin B;Li L;Li J
乙型肝炎相关性急性慢性肝功能衰竭(HBVACLF)是一种危及生命的疾病，其发生发展机制尚不清楚。本研究的目的是明确HBVACLF患者外周血单个核细胞microRNAs的特征。本研究通过高通量测序和实时定量聚合酶链式反应(qRT-PCR)验证了新的HBVACLF患者外周血单个核细胞(PBMCs)的miRNA(MiRNA)生物标志物。结果显示，78个miRNAs在慢性乙型肝炎(CHB)患者和健康对照组中的表达差异有统计学意义。在HBVACLF患者中，17个miRNAs表达水平升高或降低4倍以上，其中8个miRNAs的表达水平高于中位数。QRT-PCR验证表明，6个miRNAs(hsa-miR-21-5p、hsa-miR-34c-5p、hsa-miR-143-3p、hsa-miR-143-5p、hsa-miR-374a-3p和hsa-miR-542-3p)可作为新的诊断HBVACLF的生物标志物。检测到5个新的miRNAs(L-miR-1~5)，其中2个(L-miR-1和L-miR-3)在乙肝急性加重期肝纤维化患者中有显著差异表达。结论：HBVACLF患者PBMCs的miRNA表达谱发生了改变，6个miRNAs的特征可能成为预测HBVACLF进展的一个有前景的生物标志物。
Hepatitis B-related acute-on-chronic liver failure (HBV-ACLF) is a life-threatening condition and the mechanisms of its development and progression remain unclear. The aim of this study was to define the characteristics of peripheral blood mononuclear cell microRNAs in patients with HBV-ACLF. In this study, novel microRNA (miRNA) biomarkers of peripheral blood mononuclear cells (PBMCs) in patients with HBV-ACLF were characterised by high-throughput sequencing and validated by quantitative real-time polymerase chain reaction (qRT-PCR). The results showed 78 miRNAs were significantly differentially expressed in patients with HBV-ACLF compared to patients with chronic hepatitis B (CHB) and healthy controls. Among patients with HBV-ACLF, 17 dysregulated miRNAs increased or decreased more than 4-fold, of which eight miRNAs had higher expression levels than median level. qRT-PCR validation demonstrated that six miRNAs (hsa-miR-21-5p, hsa-miR-34c-5p, hsa-miR-143-3p, hsa-miR-143-5p, hsa-miR-374a-3p and hsa-miR-542-3p) may be useful as novel biomarkers for the diagnosis of HBV-ACLF. Five novel miRNAs (L-miR-1~5) were detected and two (L-miR-1 and L-miR-3) were significantly differentially expressed in patients with HBV-ACLF. Conclusions: The miRNA expression profile of PBMCs is altered in patients with HBV-ACLF and a signature of six miRNAs may be a promising biomarker for HBV-ACLF progression.