EARLY EVENTS IN DEVELOPMENT OF ELECTRICAL-ACTIVITY AND CONTRACTION IN EMBYRONIC RAT-HEART ASSESSED BY OPTICAL-RECORDING
EARLY EVENTS IN DEVELOPMENT OF ELECTRICAL-ACTIVITY AND CONTRACTION IN EMBYRONIC RAT-HEART ASSESSED BY OPTICAL-RECORDING
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DOI：
10.1113/jphysiol.1985.sp015897
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发表时间：
1985-01-01
影响因子：
5.5
通讯作者：
YADA, T
中科院分区：
文献类型：
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作者：
HIROTA, A;KAMINO, K;YADA, T
1. 使用电压敏感的部花青-绕丹宁染料和多元件光电二极管矩阵阵列对大鼠早期胚胎心脏的自发动作电位和收缩进行光学监测，并首次确定了大鼠心脏发生早期节律性动作电位活动的开始。 2. 自发节律动作电位首先在胚胎心脏的中央部分产生，这是在三体节发育中期，即交配后 9 1/2 天。随后，在三体节阶段末期也出现了收缩和动作电位。 3. 通常，在三体节阶段，心脏不同区域的自发动作信号是同步的。从该结果可以明显看出，在自发电活动开始时，成对的左右心脏原基完全融合。 4.在3体节胚胎心脏中，兴奋波从起搏区域以均匀的速率（0.4-0.8毫米/秒）在心脏上径向传导。然而，领跑活动的区域优先顺序并不是僵化的，而是灵活的。
1. Spontaneous action potential and contraction in the early embryonic heart of the rat have been monitored optically using a voltage-sensitive merocyanine-rhodanine dye together with a multiple-element photodiode matrix array, and the onset of rhythmical action-potential activity in the early phases of rat cardiogenesis was conclusively determined for the first time. 2. Spontaneous rhythmical action potentials were first generated in the central part of the embryonic heart at the middle period of the 3-somite stage of development, at 9 1/2 days after copulation. Subsequently, contractions coupled with the action potential also appeared at the end of the 3-somite stage. 3. Usually, at the 3-somite stage, spontaneous action signals were synchronized among the different areas in the heart. From this result, it is evident that the paired right and left cardiac primordia are fused completely at the time of initiation of spontaneous electrical activity. 4. In the 3-somite embryonic heart, excitatory waves were conducted radially over the heart, at a uniform rate (0.4-0.8 mm/s), from the pace-making area. However, the regional priority of pace-making activity is not rigid but is flexible.