Surface modification of poly-L-lactic acid fibrous scaffolds by a molecular-designed multi-walled carbon nanotube multilayer for enhancing cell interactions
Surface modification of poly-L-lactic acid fibrous scaffolds by a molecular-designed multi-walled carbon nanotube multilayer for enhancing cell interactions
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通过分子设计的多壁碳纳米管多层对聚-L-乳酸纤维支架进行表面修饰以增强细胞相互作用
DOI：
10.1016/j.carbon.2013.01.025
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发表时间：
2013-05-01
期刊：
影响因子：
10.9
通讯作者：
Xue, Wei
中科院分区：
文献类型：
--
作者：
He, Liumin;Zhao, Peipei;Xue, Wei
提出了一种用于工程细胞/碳纳米管界面的分子设计的多壁碳纳米管在聚L-乳酸电纺纤维上的逐层多层膜。通过聚乙二亚胺(PEI)与聚乳酸(PLLA)的氨解反应，在纤维表面形成稳定的带正电的单分子膜，然后交替沉积在带负电的MWCNT和带正电的壳聚糖(CS)中。热重分析表明，MWCNT在自组装过程中持续生长。多壁碳纳米管与聚阳离子之间的相互作用对多壁碳纳米管多层膜的特殊结构和性质有重要影响。MWCNT/PEI的静电相互作用减小了MWCNT之间的间距，改善了pi->；pi*相变。然而，CS链倾向于比PEI分子的链更蛇形，这可能阻碍了它的pi-和pi*转变。另一方面，拉曼分析表明，静电相互作用可能提高了MWCNT结构的无序度。MWCNT多层修饰后，支架保持了纤维状和多孔结构，并支持成纤维细胞的生长。MWCNT多层膜可诱导细胞向支架内部迁移。因此，我们创造了一种简单而有效的方法，在三维(3D)纤维支架上构建碳纳米管多层膜，以增强细胞与基质的相互作用。(C)2013爱思唯尔有限公司。保留所有权利。
We presented a molecular-designed multi-walled carbon nanotube (MWCNT) layer-by-layer (LbL) multilayer on poly-L-lactic acid (PLLA) electrospun fibers for engineering cell/CNT interfaces. A stable, positively charged monolayer was created on the fiber surface by the aminolysis reaction of poly(ethylene imine) (PEI) with PLLA, followed by alternate deposition in negatively charged MWCNT and positively charged chitosan (CS). Thermo-gravimetric analysis indicated a sustained growth of the MWCNT during the self-assembly process. The interactions between MWCNT and polycation crucially affected the specific structure and properties of the MWCNT multilayer. MWCNT/PEI electrostatic interactions reduced the gap between MWCNTs and improved the pi -> pi* transitions. However, the CS chains tended to be more serpentine than the chains of PEI molecules, which might have hindered the it pi -> pi* transitions. On the other hand, the electrostatic interactions might have enhanced the disorder grade of the MWCNT structure, as indicated by Raman analysis. The scaffolds maintained their fibrous and porous structure after MWCNT multilayer modification and supported fibroblast growth. The MWCNT multilayer induced cell migration toward the interior of the scaffolds. Therefore, we created a simple yet efficient method of building a CNT multilayer on three-dimensional (3D) fibrous scaffolds for enhancing cell-matrix interactions. (c) 2013 Elsevier Ltd. All rights reserved.