Comparative analysis of Papaver somniferum genotypes having contrasting latex and alkaloid profiles
Comparative analysis of Papaver somniferum genotypes having contrasting latex and alkaloid profiles
复制标题
DOI：
10.1007/s00709-013-0587-7
复制
发表时间：
2014-07-01
期刊：
影响因子：
2.9
通讯作者：
Khanuja, Suman P. S.
中科院分区：
文献类型：
--
作者：
Chaturvedi, Nidarshana;Singh, Mridula;Khanuja, Suman P. S.
罂粟产生治疗有用的苄基异喹啉生物碱(BIAS)，如罂粟碱、茶碱、可待因和吗啡，这些生物碱积累在其胶囊胶乳中。吗啡是一种有效的止痛剂，但也被滥用为麻醉剂，这增加了对可转化为各种镇痛剂的非麻醉性茶碱的需求。为了减少麻醉剂的威胁，已经开发出许多不同的基因类型的植物，它们缺乏吗啡和/或乳胶。Sujata是从富含生物碱的树胶收获品种Sampada发展而来的这样一种无乳胶、低生物碱生产的品种。由于其生物碱含量低、种子油营养丰富、表型无乳胶等特点，本研究开发了其在基因勘探和差异基因调控研究中的应用。对Sujata和Sampada胶囊早期和晚期的BIA分析表明，Sujata与Sampada相比，除Thebaine外，其生物碱表型均为抑制型。用消减杂交和微阵列技术对早期胶囊(高级西巴因)中的两种基因进行基于转录产物的比较分析。对一个睡眠疫霉阵列的询问产生了许多差异表达的转录本。基于同源性的注释将它们分为与乳胶有关、与油/脂有关、与生物碱有关、与细胞壁有关以及其他。这些线索将有助于描述备受追捧的Sujata表型。
Papaver somniferum produces therapeutically useful benzylisoquinoline alkaloids (BIAs) like papaverine, thebaine, codeine, and morphine that accumulate in its capsular latex. Morphine is a potent analgesic but is also abused as a narcotic, which has increased the demand for non-narcotic thebaine that can be converted into various analgesics. To curtail the narcotic menace, many distinct genotypes of the plant have been developed that are deficient in morphine and/or latex. Sujata is one such latex-less low alkaloid-producing variety developed from the alkaloid-rich gum harvest variety Sampada. Its utility for gene prospecting and studying differential gene regulation responsible for its low alkaloid, nutritive seed oil, and latex-less phenotype has been exploited in this study. BIA profiling of Sujata and Sampada capsules at the early and late stages indicated that except for thebaine, Sujata had a depressed alkaloid phenotype as compared to Sampada. Comparative transcript-based analysis of the two genotypes was carried out in the early stage capsule (higher thebaine) using subtractive hybridization and microarray. Interrogation of a P. somniferum array yielded many differentially expressing transcripts. Their homology-based annotation classified them into categories-latex related, oil/lipid related, alkaloid related, cell wall related, and others. These leads will be useful to characterize the highly sought after Sujata phenotype.