Expression and activation of epidermal growth factor receptors in meningiomas
Expression and activation of epidermal growth factor receptors in meningiomas
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脑膜瘤中表皮生长因子受体的表达和激活
DOI：
10.3171/jns.1997.87.2.0315
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发表时间：
1997-08-01
影响因子：
4.1
通讯作者：
Guha, A
中科院分区：
文献类型：
--
作者：
Carroll, RS;Black, PM;Guha, A
以往的研究表明，表皮生长因子受体（EGFR）的表达在人脑脑膜瘤。然而，EGFR的激活状态以及它们是否激活细胞质促有丝分裂信号通路尚不清楚。在这项研究中，使用北方印迹分析和聚合酶链反应，作者报告的表达表皮生长因子，转化生长因子-α，EGFR信使RNA在27脑膜瘤标本。使用Western印迹和免疫组化分析的脑膜瘤样品，作者证明，这些脑膜瘤表达的EGFR被激活。这些激活的EGFR与Shc相互作用并使其磷酸化，Shc是一种含有SH 2结构域的衔接蛋白，其在通过激活Ras信号传导途径将促有丝分裂信号从EGFR转导至细胞核中是重要的。这些结果支持了自分泌/旁分泌刺激激活人脑膜瘤EGFR可能有助于其增殖的概念。
Previous studies have demonstrated expression of epidermal growth factor receptors (EGFRs) in human cerebral meningiomas. However, the activation status of the EGFRs and whether they activate cytoplasmic mitogenic signaling pathways are not known. In this study, using Northern blot analysis and the polymerase chain reaction, the authors report expression of epidermal growth factor, transforming growth factor-alpha, and EGFR messenger RNA in 27 meningioma specimens. Using Western blot and immunohistochemical analyses of the meningioma samples, the authors demonstrate that the EGFRs expressed by these meningiomas are activated. These activated EGFRs interact with and phosphorylate Shc, an SH2 domain-containing adapter protein that is important in transducing mitogenic signals from EGFRs to the nucleus via activation of the Ras signaling pathway. These results support the concept that activation of EGFRs in human meningiomas by autocrine/paracrine stimulation may contribute to their proliferation.