Packaging of the bacteriophage lambda chromosome: a role for base sequences outside cos.
Packaging of the bacteriophage lambda chromosome: a role for base sequences outside cos.
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噬菌体 lambda 染色体的包装：cos 外部碱基序列的作用。
DOI：
10.1016/0042-6822(79)90214-9
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发表时间：
1979
期刊：
影响因子：
3.7
通讯作者：
J. Donelson
中科院分区：
文献类型：
--
作者：
M. Feiss;Richard Fisher;Deborah A. Siegele;Brian P. Nichols;J. Donelson
噬菌体λ和21不同包装特异性的遗传基础[B. Hohn, 1975，J.]。摩尔。杂志。（98, 93-106）已被检验过。一个包含21个头部基因和左染色体末端替换的λ的杂交衍生物具有21个包装特异性，因此λ和21的不同包装特异性是由于该区域的差异造成的。辅助包装实验表明，lambda和21能够在包装特异性相反的染色体上进行内聚性末端切割（cohesive end site, cos），说明共切割本身并不是导致包装特异性分化的原因。与辅助包装研究相一致的测序研究表明，λ和λ-21杂种的内聚末端位点是相同的。结果表明，22个碱基对外的碱基序列决定了λ和21的包装特性。讨论了λ和21的包装特异性可能的分子基础。lambda头体与噬菌体21的互补研究表明，只有dandfiimutants可以与21互补。由于DNA包装涉及DNA与识别蛋白和前导蛋白之间的特定相互作用，因此这两个系统之间很少有组件可互换也就不足为奇了。
The genetic basis for the divergent packaging specificities of phages λ and 21 (B. Hohn, 1975,J. Mol. Biol.,98, 93–106) has been examined. A hybrid derivative of lambda containing a substitution of the 21 head genes and left chromosome terminus has 21 packaging specificity, so the divergent packaging specificities of λ and 21 result from differences in this region. Helper packaging experiments show that lambda and 21 can carry out cohesive end site (cos) cleavage on chromosomes of the opposite packaging specificity, indicating thatcoscleavage per se is not the reason for the divergent packaging specificities. Sequencing studies, compatible with the helper packaging studies, show that the cohesive end sites of lambda and the λ-21 hybrid are identical. It is concluded that base sequences outside of the 22 base paircossegment are responsible for the packaging specifities of λ and 21. The possible molecular basis for the packaging specificities of λ and 21 is discussed. Complementation studies between lambda head ambers and phage 21 show that onlyDandFIImutants can be complemented by 21. Since DNA packaging involves specific interactions between DNA and recognition proteins and the prehead, it is not surprising that few components are interchangeable between these two systems.