Parallel human genome analysis: Microarray-based expression monitoring of 1000 genes
Parallel human genome analysis: Microarray-based expression monitoring of 1000 genes
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DOI：
10.1073/pnas.93.20.10614
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发表时间：
1996-10-01
影响因子：
11.1
通讯作者：
Davis, RW
中科院分区：
文献类型：
--
作者：
Schena, M;Shalon, D;Davis, RW
包含1046个未知序列的人类cDNA的微阵列用高速机器人打印在玻璃上。这些1.0-cm（2）DNA“芯片”用于使用高灵敏度双色杂交测定法定量监测同源人类基因的差异表达。在给定实验条件下显示差异表达模式的阵列元件通过测序进行表征。人类T细胞中已知和新的热休克和佛波酯调节基因的鉴定证明了该测定法的灵敏度。基因芯片的并行分析为大规模人类基因发现提供了一种快速有效的方法。
Microarrays containing 1046 human cDNAs of unknown sequence were printed on glass with high-speed robotics. These 1.0-cm(2) DNA ''chips'' were used to quantitatively monitor differential expression of the cognate human genes using a highly sensitive two-color hybridization assay, Array elements that displayed differential expression patterns under given experimental conditions were characterized by sequencing, The identification of known and novel heat shock and phorbol ester-regulated genes in human T cells demonstrates the sensitivity of the assay. Parallel gene analysis with microarrays provides a rapid and efficient method for large-scale human gene discovery.