Curcumin inhibited the arylamines N-acetyltransferase activity, gene expression and DNA adduct formation in human lung cancer cells (A549)
Curcumin inhibited the arylamines N-acetyltransferase activity, gene expression and DNA adduct formation in human lung cancer cells (A549)
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DOI：
10.1016/s0887-2333(03)00020-1
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发表时间：
2003-06-01
影响因子：
3.2
通讯作者：
Chung, JG
中科院分区：
文献类型：
--
作者：
Chen, YS;Ho, CC;Chung, JG
众所周知，N-乙酰转移酶（NAT）在芳胺代谢中发挥着重要作用。我们分析了 A549 人肺癌细胞对 2-氨基芴 (AF) 的 N-乙酰化对姜黄素的反应。姜黄素处理后，HPLC检测NAT活性，HPLC检测AF-DNA加合物形成，聚合酶链式反应检测NAT基因表达。姜黄素以剂量依赖性方式抑制人 A549 细胞和细胞质中的 NAT 活性。结果还表明，姜黄素可抑制和降低人肺 A549 肿瘤细胞中的基因表达（NAT1 mRNA）。将人肺 A549 肿瘤细胞与 AF 在有或没有姜黄素共处理的情况下孵育后，回收细胞并制备 DNA 并水解为核苷酸。将加合的核苷酸提取到丁醇中，并通过 HPLC 进行 AF-DNA 加合物的分析。结果还表明，姜黄素可减少人肺 A549 肿瘤细胞中 AF-DNA 加合物的形成。 (C) 2003 Elsevier Science Ltd. 保留所有权利。
It is well known that N-acetyltransferase (NAT) plays an important role in the arylamine metabolism. We analysed the response of A549 human lung cancer cells for N-acetylation of 2-aminofluorene (AF) to curcumin. After curcumin treatment, the NAT activity was examined by HPLC, AF-DNA adduct formation was examined by HPLC, and NAT gene expression by polymerase chain reaction were detected. The NAT activity in the human A549 cells and cytosols was suppressed by curcumin in a dose-dependent manner. The results also demonstrated that gene expression (NAT1 mRNA) in human lung A549 tumor cells was inhibited and decreased by curcumin. After the incubation of human lung A549 tumor cells with AF with or without curcumin co-treatment, the cells were recovered and DNA was prepared and hydrolyzed to nucleotides. The adducted nucleotides were extracted into butanol and analyzation of AF-DNA adducts was done by HPLC. The results also demonstrated that curcumin decreases AF-DNA adduct formation in the human lung A549 tumor cells. (C) 2003 Elsevier Science Ltd. All rights reserved.