Bone tissue engineering with human mesenchymal stem cell sheets constructed using magnetite nanoparticles and magnetic force
Bone tissue engineering with human mesenchymal stem cell sheets constructed using magnetite nanoparticles and magnetic force
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DOI：
10.1002/jbm.b.30752
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发表时间：
2007-08-01
影响因子：
3.4
通讯作者：
Honda, Hiroyuki
中科院分区：
文献类型：
--
作者：
Shimizu, Kazunori;Ito, Akira;Honda, Hiroyuki
不使用支架体外重建三维（31）组织可能是组织工程的替代策略。我们已经开发了一种新的组织工程策略，称为基于磁力的组织工程（Mag-TE），其中在脂质体表面带正电荷的磁铁矿阳离子脂质体（MCLs）和磁力用于构建无支架的3D组织。在这项研究中，人类骨髓间充质干细胞（MSC）磁标记与MCL接种到超低附着培养表面，和磁铁（4000 G）放置在反面。培养24小时后，MSCs形成多层片状结构。通过Mag-TE构建的片材中的MSC在使用每种诱导培养基的21天培养期后保持体外分化成成骨细胞、脂肪细胞或软骨细胞的能力。用电磁铁将Mag-TE构建的MSC片移植到裸鼠颅骨缺损处。组织学观察显示，MSC片移植后14天，在缺损区形成由成骨细胞样细胞包围的新骨，而对照组大鼠未观察到骨形成。这些结果表明，Mag-TE可以用于MSC片的移植，使用磁铁矿纳米颗粒和磁力，为骨组织工程提供了新的方法。(C)2007 Wiley Periodicals，Inc.
An in vitro reconstruction of three-dimensional (31) tissues without the use of scaffolds may be an alternative strategy for tissue engineering. We have developed a novel tissue engineering strategy, termed magnetic force-based tissue engineering (Mag-TE), in which magnetite cationic liposomes (MCLs) with a positive charge at the liposomal surface, and magnetic force were used to construct 3D tissue without scaffolds. In this study, human mesenchymal stem cells (MSCs) magnetically labeled with MCLs were seeded onto an ultralow attachment culture surface, and a magnet (4000 G) was placed on the reverse side. The MSCs formed multilayered sheet-like structures after a 24-h culture period. MSCs in the sheets constructed by Mag-TE maintained an in vitro ability to differentiate into osteoblasts, adipocytes, or chondrocytes after a 21-day culture period using each induction medium. Using an electromagnet, MSC sheets constructed by Mag-TE were harvested and transplanted into the bone defect in the crania of nude rats. Histological observation revealed that new bone surrounded by osteoblast-like cells was formed in the defect area 14 days after transplantation with MSC sheets, whereas no bone formation was observed in control rats without the transplant. These results indicated that Mag-TE could be used for the transplantation of MSC sheets using magnetite nanoparticles and magnetic force, providing novel methodology for bone tissue engineering. (C) 2007 Wiley Periodicals, Inc.