Rice oxalate oxidase gene driven by green tissue-specific promoter increases tolerance to sheath blight pathogen (Rhizoctonia solani) in transgenic rice
Rice oxalate oxidase gene driven by green tissue-specific promoter increases tolerance to sheath blight pathogen (Rhizoctonia solani) in transgenic rice
复制标题
DOI：
10.1111/mpp.12055
复制
发表时间：
2013-12-01
影响因子：
4.9
通讯作者：
Datta, Karabi
中科院分区：
文献类型：
--
作者：
Molla, Kutubuddin A.;Karmakar, Subhasis;Datta, Karabi
水稻纹枯病是由坏死性真菌枯丝核菌引起的，是水稻最具破坏性和顽固性的疾病之一，导致世界范围内水稻生产力的显著下降。在这篇文章中，为了检验稻瘟病的抗性，我们报道了以绿色组织特异性方式过度表达水稻草酸氧化酶4 （Osoxo4）基因的转基因水稻品系的产生，该基因可以分解草酸（OA），这是稻瘟病的发病因子。通过组织化学分析和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）显示，转基因植株的草酸氧化酶（OxO）活性高于非转基因对照植株。转基因水稻叶片对外源OA的耐受性优于对照水稻叶片。转基因植株对病原菌侵染有较高水平的其他防御相关基因表达。更重要的是，转基因植株对茄蚜的持久抗性显著增强。Osoxo4在水稻中过表达未表现出有害的表型效应和农艺效应。我们的研究结果表明，水稻OxO可以有效地用于植物遗传操作，以抵抗纹枯病，并可能抵抗其他由坏死性真菌引起的疾病，特别是那些分泌OA的疾病。这是首次报道在水稻中以绿色组织特异性方式表达抗纹枯病的防御基因。
Rice sheath blight, caused by the necrotrophic fungus Rhizoctonia solani, is one of the most devastating and intractable diseases of rice, leading to a significant reduction in rice productivity worldwide. In this article, in order to examine sheath blight resistance, we report the generation of transgenic rice lines overexpressing the rice oxalate oxidase 4 (Osoxo4) gene in a green tissue-specific manner which breaks down oxalic acid (OA), the pathogenesis factor secreted by R.solani. Transgenic plants showed higher enzyme activity of oxalate oxidase (OxO) than nontransgenic control plants, which was visualized by histochemical assays and sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Transgenic rice leaves were more tolerant than control rice leaves to exogenous OA. Transgenic plants showed a higher level of expression of other defence-related genes in response to pathogen infection. More importantly, transgenic plants exhibited significantly enhanced durable resistance to R.solani. The overexpression of Osoxo4 in rice did not show any detrimental phenotypic or agronomic effect. Our findings indicate that rice OxO can be utilized effectively in plant genetic manipulation for sheath blight resistance, and possibly for resistance to other diseases caused by necrotrophic fungi, especially those that secrete OA. This is the first report of the expression of defence genes in rice in a green tissue-specific manner for sheath blight resistance.