A Cross-Talk between the Erythrocyte L-Arginine/ADMA/Nitric Oxide Metabolic Pathway and the Endothelial Function in Subjects with Type 2 Diabetes Mellitus.
A Cross-Talk between the Erythrocyte L-Arginine/ADMA/Nitric Oxide Metabolic Pathway and the Endothelial Function in Subjects with Type 2 Diabetes Mellitus.
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DOI：
10.3390/nu13072306
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发表时间：
2021-07-04
期刊：
影响因子：
5.9
通讯作者：
Doroszko A
中科院分区：
文献类型：
--
作者：
Gajecki D;Gawryś J;Wiśniewski J;Fortuna P;Szahidewicz-Krupska E;Doroszko A
(1)背景：2型糖尿病（Type-2-diabetes-mellitus，DM）是最重要的心血管危险因素之一。在调节血管张力的许多分子中，一氧化氮似乎是最关键的。虽然微血管和大血管异常被广泛研究，一氧化氮代谢途径的改变需要进一步调查。此外，红细胞在血管张力调节中的作用尚未得到广泛研究。本研究的目的是评估糖尿病患者的内皮功能和红细胞和血浆中的一氧化氮代谢途径。(2)方法：采用横断面研究方法，共纳入80例受试者，其中糖尿病患者35例，健康对照者45例。评价内皮功能对不同刺激的反应。(3)结果如下：在DM组中，观察到血浆室中精氨酸和瓜氨酸浓度降低，精氨酸/ADMA和ADMA/DMA比值降低。观察到红细胞中的一氧化氮代谢得以保留，瓜氨酸水平降低，NO生物利用度显著提高。糖尿病患者对热刺激的反应表现出明显的内皮功能障碍。(4)结论：处于疾病早期阶段的DM患者在一氧化氮代谢途径中显示出显著差异，这在血浆室中更明显。红细胞构成了一种具有更高的一氧化氮生物利用度的缓冲液，受DM相关偏差的影响较小。早期糖尿病患者显示内皮功能障碍，可以使用激光多普勒血流仪进行早期诊断。
(1) Background: Type-2-diabetes-mellitus (DM) is one the most important cardiovascular-risk-factors. Among many molecules regulating vascular tone, nitric oxide appears to be the most pivotal. Although micro- and macrovascular-abnormalities are extensively studied, the alterations in the nitric-oxide-metabolic-pathway require further investigations. Additionally, the role of erythrocytes in the vascular tone regulation has not been extensively explored. The aim of this study was to evaluate the endothelial-function and the nitric-oxide-metabolic-pathway in erythrocytes and plasma of diabetic individuals. (2) Methods: A total of 80 subjects were enrolled in this cross-sectional study, including 35 patients with DM and 45 healthy individuals. The endothelial-function was evaluated in response to different stimuli. (3) Results: In the DM group, decreased Arginine and citrulline concentrations in the plasma compartment with reduced Arginine/ADMA and ADMA/DMA-ratios were observed. Preserved nitric-oxide-metabolism in erythrocytes with reduced citrulline level and significantly higher NO-bioavailability were noted. Significant endothelial dysfunction in DM individuals was proved in response to the heat-stimulus. (4) Conclusions: DM patients at an early stage of disease show significant differences in the nitric-oxide-metabolic-pathway, which are more pronounced in the plasma compartment. Erythrocytes constitute a buffer with a higher nitric-oxide-bioavailability, less affected by the DM-related deviations. Patients at an early-stage of DM reveal endothelial-dysfunction, which could be diagnosed earlier using the laser-Doppler-flowmetry.
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影响因子：
7.7
作者：
Adam, Jonathan;Brandmaier, Stefan;Wang-Sattler, Rui
通讯作者：
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影响因子：
4.4
作者：
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DOI：
10.1152/ajpheart.1995.269.5.h1613
发表时间：
1995-11-01
影响因子：
4.8
作者：
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通讯作者：
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DOI：
10.1016/j.metabol.2015.01.008
发表时间：
2015-06
期刊：
Metabolism: clinical and experimental
影响因子：
--
作者：
Irving BA;Carter RE;Soop M;Weymiller A;Syed H;Karakelides H;Bhagra S;Short KR;Tatpati L;Barazzoni R;Nair KS
通讯作者：
Nair KS
影响因子：
11.4
作者：
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