Automated analytical microarrays: a critical review.
Automated analytical microarrays: a critical review.
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DOI：
10.1007/s00216-008-2039-3
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发表时间：
2008-07
影响因子：
4.3
通讯作者：
Niessner R
中科院分区：
文献类型：
--
作者：
Seidel M;Niessner R
微阵列提供了一个强大的分析工具，在一个单一的实验中同时检测多个分析物。核酸（杂交）和抗体对抗原的特异性亲和反应是产生多重定量结果的最常见生物分析方法。基于核酸的分析仅限于检测细胞和病毒。抗体是更通用的生物分子受体，其选择性地结合小分子，如杀虫剂、小毒素和药物，以及生物聚合物（例如毒素、过敏原）和复杂的生物结构，如细菌细胞和病毒。通过产生适当的抗体，可以在多重免疫分析微阵列上检测相应的抗原分析物。食品和水分析沿着临床诊断构成了多重分析的潜在应用领域。在过去的十年中，已经开发了各种荧光、化学发光、电化学和无标记的微阵列读出系统。它们中的一些通过与流体系统组合而被构建为流通式微阵列。微阵列有可能成为一个广泛接受的分析应用系统，提供了强大的和验证的结果，在全自动化平台上成功地产生。本文综述了微阵列的自动化系统和定量多重应用的研究现状。MCR 3：全自动化学发光微阵列分析仪
Microarrays provide a powerful analytical tool for the simultaneous detection of multiple analytes in a single experiment. The specific affinity reaction of nucleic acids (hybridization) and antibodies towards antigens is the most common bioanalytical method for generating multiplexed quantitative results. Nucleic acid-based analysis is restricted to the detection of cells and viruses. Antibodies are more universal biomolecular receptors that selectively bind small molecules such as pesticides, small toxins, and pharmaceuticals and to biopolymers (e.g. toxins, allergens) and complex biological structures like bacterial cells and viruses. By producing an appropriate antibody, the corresponding antigenic analyte can be detected on a multiplexed immunoanalytical microarray. Food and water analysis along with clinical diagnostics constitute potential application fields for multiplexed analysis. Diverse fluorescence, chemiluminescence, electrochemical, and label-free microarray readout systems have been developed in the last decade. Some of them are constructed as flow-through microarrays by combination with a fluidic system. Microarrays have the potential to become widely accepted as a system for analytical applications, provided that robust and validated results on fully automated platforms are successfully generated. This review gives an overview of the current research on microarrays with the focus on automated systems and quantitative multiplexed applications. MCR 3: A fully automated chemiluminescence microarray reader for analytical microarrays
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DOI：
10.1128/cdli.12.8.959-969.2005
发表时间：
2005-08-01
期刊：
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
影响因子：
--
作者：
Dias, D;Van Doren, J;Esser, MT
通讯作者：
Esser, MT
影响因子：
2.2
作者：
Belleville, E;Dufva, M;Christensen, CBV
通讯作者：
Christensen, CBV
影响因子：
2.7
作者：
Belleville, E;Dufva, M;Christensen, CBV
通讯作者：
Christensen, CBV
影响因子：
7.4
作者：
Ahn, S;Walt, DR
通讯作者：
Walt, DR
影响因子：
14.9
作者：
Barnes M;Freudenberg J;Thompson S;Aronow B;Pavlidis P
通讯作者：
Pavlidis P