Black tea and D. candidum extracts play estrogenic activity via estrogen receptor α-dependent signaling pathway
Black tea and D. candidum extracts play estrogenic activity via estrogen receptor α-dependent signaling pathway
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红茶和白花石竹提取物通过雌激素受体α依赖性信号通路发挥雌激素活性
DOI：
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Sheng J
中科院分区：
文献类型：
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作者：
Wang Yogsen;Sun Jing;Hu Xin;Fu Xueqi;Zhang Kun;Sun Yuchu;Sheng Jun;Fu XQ;Sheng J
近年来，植物雌激素已被证明是有用的选择性雌激素受体调节剂。红茶（BT）和D.然而，在很大程度上，仍然难以找到铁皮石斛（DC）以及这两种草药的组合。本研究旨在探讨BT和DC提取物的植物雌激素样作用及其可能的机制。采用MTT法检测其对T47 D（ER+细胞株）增殖的影响。用流式细胞仪测定ER+细胞S期比例。雌激素拮抗剂ICI 182，780阻断ER功能。Western blot检测ER介导的PI 3 K/AKT和ERK信号通路的激活。Western blot和实时定量PCR检测ERα、PGR、PS2和Cyclin D1的表达。首先，我们的研究结果发现BT和DC提取物促进ER阳性细胞的细胞增殖，并且这种作用是ER依赖性的。此外，BT和DC提取物增加S期细胞数量。其次，ER以下的PI 3 K、AKT和ERK通路被植物雌激素处理激活，并且这种激活被ER拮抗剂阻断。此外，延长BT和DC处理增加了ESR 1和PGR的表达。因此，不仅ESR 1和PGR的mRNA水平，而且雌激素依赖性效应物ps2和细胞周期蛋白D1的mRNA水平也被植物雌激素增加，并被ICI 182，780阻断。综合来看，BT和DC提取物均具有植物雌激素样作用，这为植物雌激素的开发和应用提供了新的思路和实验依据。
In recent years, phytoestrogens have been shown as useful selective estrogen receptor modulators. The .estrogen-like effects of black tea (BT) and D. candidum (DC), as well as the combination of the two herbs, have .remained largely elusive. This study aims to investigate the phytoestrogenic effect of BT and DC extract, and the .possible mechanism. The effects on T47D (ER+ cell line) proliferation were evaluated by using MTT assay. The S .phase proportion of ER+ cells was determined by using flow cytometry. The estrogen antagonist ICI 182,780 was .applied to block the ER function. The activation of ER-mediated PI3K/AKT and ERK signal pathways were observed .by using western blot. Expression of ERα and PGR, as well as PS2 and Cyclin D1 were detected by using western .blot and real-time quantitative PCR. Firstly, our results found that BT and DC extracts promoted cell proliferation in .ER-positive cells, and this effect was ER-dependent. Besides, BT and DC extracts increased the S-phase cell number. Next, PI3K, AKT and ERK pathways below ER were activated by phytoestrogen treatment, and this activation .was blocked by the ER antagonist. Moreover, prolonged BT and DC treatments increased the expression of ESR1 .and PGR. Consistently, the mRNA levels of not only ESR1 and PGR but also estrogen-dependent effectors ps2 and .cyclin D1, were increased by phytoestrogens and blocked by ICI 182,780. Taken Together, BT and DC extracts have .phytoestrogenic effects, and this may provide new ideas and experimental basis for the development and application of phytoestrogens.