Individual variation in buffalo somatic cell cloning efficiency is related to glycolytic metabolism
Individual variation in buffalo somatic cell cloning efficiency is related to glycolytic metabolism
复制标题
水牛体细胞克隆效率的个体差异与糖酵解代谢有关
DOI：
10.1007/s11427-021-2039-6
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发表时间：
2022-03
期刊：
影响因子：
--
通讯作者：
Deshun Shi
中科院分区：
文献类型：
--
作者：
Chan Luo;Zhiqiang Wang;Jinling Wang;Feng Yun;Fenghua Lu;Jiayuan Fu;Qingyou Liu;Deshun Shi
哺乳动物个体的体细胞克隆效率不同，但导致这种变化的机制知之甚少。本研究发现，克隆效率高的布法罗胎儿成纤维细胞（BFF）能量代谢旺盛，染色质结构疏松，H3 K9乙酰化程度高，异染色质蛋白1α（HP 1 α）表达低。ChIP-seq显示，克隆效率高的BFF在糖酵解基因上游附近有更多的H3 K9 ac区域，ATAC-seq显示，BFF涉及更多与糖酵解基因相关的开放位点。通过qRT-PCR还发现这些糖酵解基因的表达在高克隆效率BFF中更高。糖酵解的两个关键酶PDKs和LDH被证实与BFF的组蛋白乙酰化和染色质开放有关。用PDK 1激活剂PS48处理低克隆效率的BFF，可使细胞内乳酸生成量和H3 K9乙酰化水平增加，组蛋白去乙酰化酶活性和HP 1 α表达降低，染色质结构减少，克隆胚发育为囊胚的比例增加。这些结果表明，布法罗体细胞克隆效率与其糖酵解代谢和染色质结构有关，提高糖酵解代谢可以提高克隆效率。
Mammalian individuals differ in their somatic cell cloning efficiency, but the mechanisms leading to this variation is poorly understood. Here we found that high cloning efficiency buffalo fetal fibroblasts (BFFs) displayed robust energy metabolism, looser chromatin structure, high H3K9 acetylation and low heterochromatin protein 1α (HP1α) expression. High cloning efficiency BFFs had more H3K9ac regions near to the upstream of glycolysis genes by ChIP-seq, and involved more openness loci related to glycolysis genes through ATAC-seq. The expression of these glycolysis genes was also found to be higher in high cloning efficiency BFFs by qRT-PCR. Two key enzymes of glycolysis, PDKs and LDH, were confirmed to be associated with histone acetylation and chromatin openness of BFFs. Treatment of low cloning efficiency BFFs with PS48 (activator of PDK1) resulted in an increase in the intracellular lactate production and H3K9 acetylation, decrease in histone deacetylase activity and HP1α expression, less condensed chromatin structure and more cloning embryos developing to blastocysts. These results indicate that the cloning efficiency of buffalo somatic cells is associated with their glycolytic metabolism and chromatin structure, and can be improved by increasing glycolytic metabolism.
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影响因子：
3.7
作者：
Tao Wang;Matthew V. Holt;N. L. Young
通讯作者：
Tao Wang;Matthew V. Holt;N. L. Young
影响因子：
2.8
作者：
Luo, Chan;Lu, Fenghua;Shi, Deshun
通讯作者：
Shi, Deshun
DOI：
10.1126/science.1227166
发表时间：
2013-01-11
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Shimazu T;Hirschey MD;Newman J;He W;Shirakawa K;Le Moan N;Grueter CA;Lim H;Saunders LR;Stevens RD;Newgard CB;Farese RV Jr;de Cabo R;Ulrich S;Akassoglou K;Verdin E
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Verdin E
Warburg效应决定了丁酸酯介导的组蛋白乙酰化和细胞增殖的机制。
影响因子：
16
作者：
Donohoe, Dallas R.;Collins, Leonard B.;Wali, Aminah;Bigler, Rebecca;Sun, Wei;Bultman, Scott J.
通讯作者：
Bultman, Scott J.
DOI：
--
发表时间：
2007
期刊：
--
影响因子：
--
作者：
D. Shi;F. Lu;Yingming Wei;K. Cui;Sufang Yang;Jingwei Wei;Qingyou Liu
通讯作者：
D. Shi;F. Lu;Yingming Wei;K. Cui;Sufang Yang;Jingwei Wei;Qingyou Liu