Chondrogenic differentiation of adipose-derived adult stem cells in agarose, alginate, and gelatin scaffolds
Chondrogenic differentiation of adipose-derived adult stem cells in agarose, alginate, and gelatin scaffolds
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DOI：
10.1016/j.biomaterials.2003.10.045
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发表时间：
2004-07-01
期刊：
影响因子：
14
通讯作者：
Guilak, F
中科院分区：
文献类型：
--
作者：
Awad, HA;Wickham, MQ;Guilak, F
假设工程组织结构中成年干细胞的分化和生长受细胞生物材料相互作用的影响。在这项研究中，我们比较了在藻酸盐和琼脂糖水凝胶中播种的人脂肪衍生的成年茎（HADAS）细胞以及多孔明胶支架（Surgifoam）以及组织工程软骨构建体的功能性能。与对照条件相比，含有转化生长因子β的软骨培养基显着提高了蛋白质和蛋白聚糖合成的速率以及DNA，硫酸糖胺聚糖和羟基的含量。此外，软骨培养条件导致86％。 160％增加（P
The differentiation and growth of adult stem cells within engineered tissue constructs are hypothesized to be influenced by cell-biomaterial interactions. In this study, we compared the chondrogenic differentiation of human adipose-derived adult stem (hADAS) cells seeded in alginate and agarose hydrogels, and porous gelatin scaffolds (Surgifoam), as well as the functional properties of tissue engineered cartilage constructs. Chondrogenic media containing transforming growth factor beta I significantly increased the rates of protein and proteoglycan synthesis as well as the content of DNA, sulfated glycosaminoglycans, and hydroxyproline of engineered constructs as compared to control conditions. Furthermore, chondrogenic culture conditions resulted in 86%. and 160% increases (p