TTX blocks baclofen-induced phase shifts of the mammalian circadian pacemaker in vitro.
TTX blocks baclofen-induced phase shifts of the mammalian circadian pacemaker in vitro.
复制标题
TTX 在体外阻断巴氯芬诱导的哺乳动物昼夜节律起搏器的相移。
DOI：
10.1016/s0006-8993(99)01791-6
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发表时间：
1999
期刊：
影响因子：
2.9
通讯作者：
Prosser,RA
中科院分区：
文献类型：
--
作者：
Bergeron,HE;Danielson,B;Biggs,KR;Prosser,RA
哺乳动物的昼夜节律起搏器，位于视交叉上核（SCN），表达24小时的节奏时，在体外分离。GABAA激动剂蝇蕈醇在中间主观日期间诱导相位提前，而GABAB激动剂巴氯芬诱导白天相位提前和夜间相位延迟。在这里，我们提出的证据表明，河豚毒素（TTX）完全阻断细菌诱导的相移在体外，但不阻止蝇蕈醇诱导的体外相位提前。这些结果表明，GABAA，而不是GABAB，受体位于SCN起搏细胞。
The mammalian circadian pacemaker, located in the suprachiasmatic nucleus (SCN), expresses 24-h rhythms when isolated in vitro. The GABAAagonist, muscimol, induces phase advances during the mid-subjective day, while the GABABagonist, baclofen, induces both daytime phase advances and nighttime phase delays. Here, we present evidence that tetrodotoxin (TTX) completely blocks baclofen-induced phase shifts in vitro, but does not block in vitro phase advances induced by muscimol. These results suggest that GABAA, but not GABAB, receptors are located on SCN pacemaker cells.