Angiopoietin-1, angiopoietin-2 and tie-2 in the coronary circulation of patients with and without coronary collateral vessels.
Angiopoietin-1, angiopoietin-2 and tie-2 in the coronary circulation of patients with and without coronary collateral vessels.
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DOI：
10.1253/circj.71.343
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发表时间：
2007-02
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通讯作者：
W. Mitsuma;M. Kodama;S. Hirono;M. Ito;M. Ramadan;Komei Tanaka;Makoto Hoyano;T. Saigawa;T. Kashimura;K. Fuse;Y. Okura;Y. Aizawa
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文献类型：
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作者：
W. Mitsuma;M. Kodama;S. Hirono;M. Ito;M. Ramadan;Komei Tanaka;Makoto Hoyano;T. Saigawa;T. Kashimura;K. Fuse;Y. Okura;Y. Aizawa
背景血管生成素（Ang）/Tie-2系统在冠状动脉侧枝生长中的作用尚不清楚，因此本研究的目的是调查和阐明该系统与冠状动脉疾病（CAD）患者冠状动脉侧枝形成的关系。方法和结果 招募了 59 名 CAD 患者。心导管插入术中采集左心室（LV）和冠状窦（CS）血样，并通过酶联免疫吸附测定法测定血清Ang-1、Ang-2和Tie-2浓度。然后将患者分为轻度 CAD (n=30)（定义为冠状动脉管腔直径狭窄 </=90%）或重度 CAD（n=29），即冠状动脉完全（或接近完全）闭塞，需要冠状动脉侧支生长。 LV 和 CS 血清中的 Ang-1、Ang-2 和 Tie-2 在各组之间没有显着差异。在严重CAD组中，与轻度CAD组相比，发现冠脉循环中Tie-2的溢出（CS-LV值）（3.43+/-2.22 vs -3.29+/-1.54 ng/ml，p=0.01），而Ang-1和Ang-2的CS-LV值在组间没有差异。在侧支循环发达的患者中，Tie-2 的产生显着增加。冠状动脉 Ang-2 和 Tie-2 水平之间存在显着正相关（r=0.44，p<0.001）。结论 Tie-2 可能是在冠状动脉循环中产生的，并且可能诱导 CAD 患者冠状动脉侧支循环的发育或维持。此外，Ang-2在冠状动脉侧枝形成中的作用可能比Ang-1更重要。
BACKGROUND The role of the angiopoietin (Ang)/Tie-2 system in coronary collateral growth is not well understood, so the purpose of this study was to investigate and elucidate the relationship of this system to coronary collateral formation in patients with coronary artery disease (CAD). METHODS AND RESULTS Fifty-nine patients with CAD were recruited. Blood samples from the left ventricle (LV) and coronary sinus (CS) were obtained during cardiac catheterization, and serum concentrations of Ang-1, Ang-2, and Tie-2 were measured by enzyme-linked immunosorbent assay. Patients were then classified as mild CAD (n=30), defined as </=90% stenosis of the coronary arterial luminal diameter, or severe CAD (n=29), which was total (or near total) coronary occlusion requiring coronary collateral growth. Ang-1, Ang-2, and Tie-2 in the LV and CS sera were not significantly different between groups. In the severe CAD group, spillover of Tie-2 (CS-LV value) from the coronary circulation was found in comparison with the mild CAD group (3.43+/-2.22 vs -3.29+/-1.54 ng/ml, p=0.01), whereas the CS-LV values of Ang-1 and Ang-2 did not differ between groups. Tie-2 production was markedly increased in patients with well-developed collaterals. A positive and significant correlation was found between coronary Ang-2 and Tie-2 levels (r=0.44, p<0.001). CONCLUSIONS Tie-2 is probably produced in the coronary circulation and may induce the development or maintenance of coronary collaterals in CAD patients. Furthermore, the role of Ang-2 in the formation of coronary collaterals may be more important than that of Ang-1.