The influence of laminin-derived peptides conjugated to Lys-capped PLLA on neonatal mouse cerebellum C17.2 stem cells
The influence of laminin-derived peptides conjugated to Lys-capped PLLA on neonatal mouse cerebellum C17.2 stem cells
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层粘连蛋白衍生肽与 Lys 加帽 PLLA 缀合对新生小鼠小脑 C17.2 干细胞的影响
DOI：
10.1016/j.biomaterials.2008.12.020
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发表时间：
2009-03-01
期刊：
影响因子：
14
通讯作者：
Lu, Jiang
中科院分区：
文献类型：
--
作者：
He, Liumin;Liao, Susan;Lu, Jiang
化学引导信号被用来刺激神经元粘附和神经突生长。在本研究中，氨基官能化的PLLA，赖氨酸封端的PLLA [K-（CH 2）（n）-PLLA（n = 2，5. 8）]，合成了赖氨酸分子和PLLA骨架之间具有不同长度的连接空间。由K-（CH 2）（n）-PLLA/PLLA共混物（10/90，w/w）制备滴铸膜，并且在所得膜的表面上检测到氨基。水热处理后，薄膜表面胺基增多，亲水性明显提高。代表性的原子力显微镜（AFM）图像表明，掺入赖氨酸封端的PLLA到PLLA矩阵增加了膜的粗糙度，并导致在相分离与不同的两个纳米域，这可能对应于亲水和疏水域。此外，层粘连蛋白衍生肽CYIGSR（Cys-Tyr-IIe-Gly-Ser-Arg）和CSIKVAV（Cys-Ser-IIe-Lys-Val-Ala-瓦尔）通过连接试剂磺基琥珀酰亚胺基4-（N-马来酰亚胺甲基）环己烷-1-羧酸酯（Suifo-SMCC）共同连接到赖氨酸封端的PLLA的胺基。将新生小鼠小脑C17.2干细胞接种在肽接枝的K-（CH_2）（n）-PLLA/PLLA（n = 2，5，8）膜上，并以纯PLLA膜作为对照。在培养期间，在肽接枝膜上获得比PLLA膜更好的存活力和更长的神经突，特别是对于K-（CH 2）（5）-PLLA/PLLA，其具有最高的肽密度0.28 +/- 0.03 μ g/cm（2）。这项研究突出了使用赖氨酸帽状PLLA与层粘连蛋白衍生肽促进神经再生的潜力。(C)2008爱思唯尔有限公司保留所有权利。
Chemical guiding cues are being exploited to stimulate neuron adhesion and neurite outgrowth. In this Study, an amino-functioned PLLA, lysine-capped PLLA [K-(CH2)(n)-PLLA (n = 2, 5. 8)], was synthesized with different length of linking spaces between lysine molecule and PLLA backbone. Drop-cast films were fabricated from K-(CH2)(n)-PLLA/PLLA blends (10/90, w/w) and amino groups were detected on the surfaces of the resultant films. More amine groups were detected on the surface and the hydrophilicity of the films was obviously improved by annealing the films in water. The representative atomic force microscopy (AFM) images indicated that incorporation of lysine-capped PLLA into PLLA matrix increased the roughness of the films and resulted in a phase separation with distinct two nano-domains which may correspond to the hydrophilic and hydrophobic domains. Furthermore, the laminin-derived peptides, CYIGSR (Cys-Tyr-IIe-Gly-Ser-Arg) and CSIKVAV (Cys-Ser-IIe-Lys-Val-Ala-Val), were jointly tethered to the amine groups of lysine-capped PLLA by a linking reagent sulfo-succinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate (Suifo-SMCC). The neonatal Mouse cerebellum C 17.2 stem cells were seeded on the peptides-grafted K-(CH2)(n)-PLLA/PLLA (n = 2, 5, 8) films and pure PLLA films were Used as controls. Improved viability and longer neurites were obtained on the peptide-grafted films than PLLA film over the cultivation period, especially for K-(CH2)(5)-PLLA/PLLA, which had the highest peptide density of 0.28 +/- 0.03 mu g/cm(2). This study highlights the potential of using the lysine-cappecled PLLA with laminin-derived peptides for promoting nerve regeneration. (C) 2008 Elsevier Ltd. All rights reserved.