Sexual plasticity of ovarian germ cells in rainbow trout
Sexual plasticity of ovarian germ cells in rainbow trout
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DOI：
10.1242/dev.044982
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发表时间：
2010-04-15
期刊：
影响因子：
4.6
通讯作者：
Okutsu, Tomoyuki
中科院分区：
文献类型：
--
作者：
Yoshizaki, Goro;Ichikawa, Masaki;Okutsu, Tomoyuki
鱼类性腺的性可塑性在性分化期后下降；然而，关于性分化后生殖细胞本身的可塑性的信息有限。最近，我们利用虹鲑鱼(Oncorhynchus MykISS)建立了一种新的生殖细胞移植系统，该系统为分析配子发生的发育和细胞机制提供了一个独特的平台。使用这项技术，我们在这里展示了从6到9个月大的捐赠者分离出来的移植的卵巢生殖细胞可以定植于性未分化的胚胎性腺并恢复配子发生。将含有卵原细胞的卵巢生殖细胞和分离自雌性虹鱼的早期卵母细胞移植到孵化期雌雄鱼苗的腹膜腔内，观察供体细胞的行为。移植的卵巢生殖细胞向受者性腺迁移，与胚胎性腺体细胞相互作用，迅速增殖，最终分化为卵子和精子。此外，从受体鱼获得的捐赠者衍生的卵子和精子具有功能，并能够产生正常的后代。这些发现表明，有丝分裂生殖细胞，即卵原细胞，具有高度的性可塑性。
The sexual plasticity of fish gonads declines after the sex differentiation period; however, information about the plasticity of the germ cells themselves after sex differentiation is limited. Using rainbow trout (Oncorhynchus mykiss), we recently established a novel germ cell transplantation system that provides a unique platform with which to dissect the developmental and cellular mechanisms underlying gametogenesis. Using this technique, we show here that transplanted ovarian germ cells isolated from 6-to 9-month-old donors can colonize sexually undifferentiated embryonic gonads and resume gametogenesis. Ovarian germ cells containing oogonia and early oocytes isolated from female rainbow trout were transplanted into the peritoneal cavities of hatching-stage fry of both sexes and the behavior of the donor cells was observed. The transplanted ovarian germ cells migrated towards the recipient gonads, interacted with embryonic gonadal somatic cells, proliferated rapidly, and eventually differentiated into eggs in female recipients and sperm in male recipients. Furthermore, the donor-derived eggs and sperm obtained from the recipient fish were functional and were able to produce normal offspring. These findings indicate that mitotic germ cells, the oogonia, possess a high level of sexual plasticity.