STRAND DISSOCIATION AND COOPERATIVE MELTING OF DOUBLE-STRANDED DNAS DETECTED BY DENATURANT GRADIENT GEL-ELECTROPHORESIS
STRAND DISSOCIATION AND COOPERATIVE MELTING OF DOUBLE-STRANDED DNAS DETECTED BY DENATURANT GRADIENT GEL-ELECTROPHORESIS
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DOI：
10.1093/oxfordjournals.jbchem.a134651
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发表时间：
1984-01-01
影响因子：
2.7
通讯作者：
TANAKA, T
中科院分区：
文献类型：
--
作者：
NISHIGAKI, K;HUSIMI, Y;TANAKA, T
使用由Fischer和Lerman设计的变性剂梯度凝胶电泳的精确分析被发现呈现双链DNA片段的精细结构的特定模式。这些似乎是由特定变性过程引起的DNA片段的迁移率变化。变性剂对DNA解链的影响与温度的影响相似。观察到的模式，在与理论上获得的DNA的熔解过程相比，DNA熔解和链解离密切相关。一些电泳迁移率的转变显示了延迟对应于每个合作熔化。链离解发生在理论预测的条件下。DNA片段的电泳延迟程度似乎与熔化区域的大小相对应。实验结果表明，该方法在DNA稳定性图谱的研究中具有传统方法无法比拟的优越性。
Precise analysis using the denaturant gradient gel electrophoresis which was devised by Fischer and Lerman, was found to present specific patterns of fine structures for double stranded DNA fragments. These seem to be a mobility change of DNA fragments caused by specific denaturation processes. The effect of denaturants on DNA melting was ascertained to be similar to the effect of temperature. The observed patterns, in comparison with the melting processes of DNA theoretically obtained, were closely related to DNA meltings and strand dissociations. A number of electrophoretic mobility transitions showed the retardation correspondent to each of the cooperative meltings. Strand dissociations occurred under the conditions theoretically predicted. The degree of retardation in electrophoresis for DNA fragments seemed to correspond to the size of melted regions. Methods presented here were proved to have advantages over the conventional ones for the study of DNA stability maps.