Application of high-intensity focused ultrasound for umbilical artery occlusion in a rabbit model
Application of high-intensity focused ultrasound for umbilical artery occlusion in a rabbit model
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DOI：
10.1002/uog.4008
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发表时间：
2007-07-01
影响因子：
7.1
通讯作者：
Kushima, M.
中科院分区：
文献类型：
--
作者：
Ichizuka, K.;Ando, S.;Kushima, M.
目的探讨高强度聚焦超声(HIFU)在兔胎儿脐动脉血流阻断中的应用。利用该换能器，于妊娠第25天经母兔腹部皮肤以1.4、2.75或5.5kW/cm(2)的功率对4只时间交配的日本大白兔(11胎)的胎儿腹内脐动脉进行HIFU照射。采用5-S高强度聚焦超声照射，直至超声心动图证实脐带血流停止和心脏骤停。结果胎儿脐动脉直径平均为0.6±-0.2 mm，平均峰值血流速度为44.7+/-18.5 cm/S，当照射功率为5.5kW/cm2时，15个疗程内血流完全闭塞。结论HIFU可阻断胎兔脐动脉血流。版权所有(C)2007 ISUOG。作者：John Wiley&Sons，Ltd.
Objectives To investigate the application of high-intensity focused ultrasound (HIFU) for fetal umbilical artery blood flow occlusion in a rabbit model.Methods A prototype HIFU transducer in combination with an imaging probe with Doppler capability was constructed. Using this transducer, HIFU was applied at 1.4, 2.75 or 5.5 kW/cm(2) through the maternal abdominal skin to the fetal intra-abdominal umbilical arteries of four time-mated Japanese White rabbits (11 fetuses) on gestational day 25. Courses of 5-s HIFU exposure were performed until cessation of umbilical blood flow and cardiac arrest were confirmed by Doppler ultrasonograpby. Fetal necropsy was performed and exposed lesions were assessed by microscopic histological analysis.Results The mean diameter of the fetal umbilical artery was 0.6 +/- 0.2 mm and the mean peak systolic velocity of arterial blood flow was 44.7 +/- 18.5 cm/s. When HIFU was applied at 5.5 kW/cm(2), blood flow was completely occluded within 15 courses. HIFU exposure brought about vacuolar degeneration and destruction of elastic fibers in the tunica media of the artery.Conclusions HIFU can be used to occlude umbilical artery blood flow in fetal rabbits. Copyright (C) 2007 ISUOG. Published by John Wiley & Sons, Ltd.