WEAK-ACID UNCOUPLERS OF OXIDATIVE PHOSPHORYLATION - MECHANISM OF ACTION ON THIN LIPID MEMBRANES
WEAK-ACID UNCOUPLERS OF OXIDATIVE PHOSPHORYLATION - MECHANISM OF ACTION ON THIN LIPID MEMBRANES
复制标题
DOI：
10.1016/0005-2728(70)90055-1
复制
发表时间：
1970-01-01
期刊：
影响因子：
--
通讯作者：
FINKELST.A
中科院分区：
文献类型：
--
作者：
FINKELST.A
氧化磷酸化的弱酸解偶联剂如asp-trifluoromethoxycarbonylcyanidephenylhydrazone、tetracloro-2-trifluoromethyl benzimidazole、m-chlorocarbonylcyanidephenylhydrazone和2，4-dinitrophenol可使薄脂质膜的电导增加几个数量级。在这种高电导状态下，这些膜似乎理想地选择性渗透H+或OH−。然而，我们认为，膜中的主要电荷载体既不是H+也不是OH−，而是在未解离和解离形式的弱酸之间形成的二聚体，并且我们表明，关于这些弱酸对薄脂质膜的作用的所有数据都与这一图像一致。
Weak-acid uncouplers of oxidative phosphorylation such asp-trifluoromethoxycarbonylcyanidephenylhydrazone, tetrachloro-2-trifluoromethylbenzimidazole,m-chlorocarbonylcyanidephenylhydrazone, and 2,4-dinitrophenol can increase the conductance of thin lipid membranes by several orders of magnitude. In this high conductance state these membranes appear to be ideally selectively permeable to H+or OH−. We suggest, however, that the primary charge carrier in the membrane is neither H+or OH−, but rather a dimer formed between the undissociated and dissociated form of the weak acid, and we show that all of the data on the action of these weak acids on thin lipid membranes are consistent with this picture.