Suppression of Virulent Porcine Epidemic Diarrhea Virus Proliferation by the PI3K/Akt/GSK-3α/β Pathway.
Suppression of Virulent Porcine Epidemic Diarrhea Virus Proliferation by the PI3K/Akt/GSK-3α/β Pathway.
复制标题
通过 PI3K/Akt/GSK-3α/β 途径抑制猪流行性腹泻病毒的增殖
DOI：
10.1371/journal.pone.0161508
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Tong G
中科院分区：
文献类型：
--
作者：
Kong N;Wu Y;Meng Q;Wang Z;Zuo Y;Pan X;Tong W;Zheng H;Li G;Yang S;Yu H;Zhou EM;Shan T;Tong G
Porcine epidemic diarrhea virus (PEDV) has recently caused high mortality in suckling piglets with subsequent large economic losses to the swine industry. Many intracellular signaling pathways, including the phosphatidylinositol 3-kinase (PI3K)/Akt pathway, are activated by viral infection. The PI3K/Akt pathway is an important cellular pathway that has been shown to be required for virus replication. In the present study, we found that the PEDV JS-2013 strain activated Akt in Vero cells at early (5–15 min) and late stages (8–10 h) of infection. Inhibiting PI3K, an upstream activator of Akt, enhanced PEDV replication. Inhibiting GSK-3α/β, one of the downstream effectors of PI3K/Akt pathway and regulated by Akt during PEDV infected Vero cells, also enhanced PEDV replication. Collectively, our data suggest that PI3K/Akt/GSK-3α/β signaling pathway is activated by PEDV and functions in inhibiting PEDV replication.
