Polymer Dynamics, Fluorescence Correlation Spectroscopy, and the Limits of Optical Resolution
Polymer Dynamics, Fluorescence Correlation Spectroscopy, and the Limits of Optical Resolution
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DOI：
10.1103/physrevlett.108.108101
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发表时间：
2012-03-07
影响因子：
8.6
通讯作者：
Enderlein, Joerg
中科院分区：
文献类型：
--
作者：
Enderlein, Joerg
近年来，荧光相关光谱越来越多地应用于纳米尺度聚合物动力学的研究。核心思想是从测量的自相关曲线中提取有效的均方位移函数，其中包含有关基础构象动力学的信息。这封信介绍了荧光相关光谱在纳米级构象和扩散动力学研究中的适用性的基础研究。我们发现荧光相关光谱无法可靠地阐明远小于所用光学器件分辨率极限的长度尺度上的过程，并且其不当使用可能会产生观察到的动力学的虚假结果。
In recent years, fluorescence correlation spectroscopy has been increasingly applied for the study of polymer dynamics on the nanometer scale. The core idea is to extract, from a measured autocorrelation curve, an effective mean-square displacement function that contains information about the underlying conformational dynamics. This Letter presents a fundamental study of the applicability of fluorescence correlation spectroscopy for the investigation of nanoscale conformational and diffusional dynamics. We find that fluorescence correlation spectroscopy cannot reliably elucidate processes on length scales much smaller than the resolution limit of the optics used and that its improper use can yield spurious results for the observed dynamics.