Chitosan based Pickering emulsion as a promising approach for topical application of rutin in a solubilized form intended for wound healing: In vitro and in vivo study
Chitosan based Pickering emulsion as a promising approach for topical application of rutin in a solubilized form intended for wound healing: In vitro and in vivo study
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DOI：
10.1016/j.ijpharm.2017.10.044
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发表时间：
2017-12-20
影响因子：
5.8
通讯作者：
Salama, Abeer A. A.
中科院分区：
文献类型：
--
作者：
Asfour, Marwa Hasanein;Elmotasem, Heba;Salama, Abeer A. A.
本研究的目的是通过将疏水药物芦丁负载到由自聚集壳聚糖颗粒(SACP)稳定的新型Pickering乳剂中，以可溶的形式制备用于伤口愈合的药物芦丁。采用高速均质机制备芦丁Pickering乳剂。通过跌落试验、光学显微镜、液滴大小和Zeta电位测定对其进行了表征。结果表明，SACP为纳米级，接触角为42.47+/-1.19度，有利于O/W乳液的稳定。长期稳定性研究表明，油相含量为20%和30%的配方在5个月内稳定性较好，Zeta电位为-48.1+/-4.7~-78.4+/-4.1 mV，液滴尺寸略有增大，稳定性较好。24小时内芦丁释放率接近100%。用所选配方治疗白化大鼠创面10天，几乎完全愈合。除组织病理学检查外，还进行氧化应激标志物、透明质酸和I型胶原的生化分析。结果表明，芦丁以可溶形式缓释，以及所制备的Pickering乳剂中其他组分的协同作用，可能具有潜在的伤口愈合作用。
The aim of the present study is to formulate the hydrophobic drug rutin in a solubilized form, intended for wound healing, via its loading into a novel Pickering emulsion stabilized by self aggregated chitosan particles (SACP). Rutin-loaded Pickering emulsion formulae were prepared using a high speed homogenizer. They were characterized by drop test, optical microscopy, droplet size and zeta potential determination. The results revealed that SACP have a nano size and a contact angle of 42.47 +/- 1.19 degrees that tend to stabilize O/W emulsion. The droplet size of all investigated formulations ranged between 5.8 +/- 1.1 and 18.7 +/- 3.4 mu m. The long term stability study revealed that formulae containing 20% and 30% oily phase were stable against coalescence, the droplet size was slightly increased with zeta potential ranged from -48.1 +/- 4.7 to -78.4 +/- 4.1 mV, during the storage period up to 5 months, indicating good stability. The release of rutin was almost 100% within 24 h. Treatment of the wounded skin tissue of the Albino rats with the selected formula, for ten days, revealed almost complete healing. Biochemical analysis for oxidative stress markers, hyaluronic acid and collagen type I in addition to histopathological study were performed. The results suggested that the sustained release of rutin in a solubilized form as well as the synergistic effect of other components of the prepared Pickering emulsion could have a potential wound healing effect.