THE QUANTIFICATION OF HUMAN-IGG SUBCLASSES IN REFERENCE PREPARATIONS
THE QUANTIFICATION OF HUMAN-IGG SUBCLASSES IN REFERENCE PREPARATIONS
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DOI：
10.1016/0009-8981(85)90262-1
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发表时间：
1985-01-01
影响因子：
5
通讯作者：
DUIMEL, WJ
中科院分区：
文献类型：
--
作者：
KLEIN, F;SKVARIL, F;DUIMEL, WJ
Three laboratories took part in an investigation about the possibilities of quantitative determination of IgG subclasses. Polyclonal monospecific antisera were used in combination with different kinds of calibrators to assess the IgG subclass levels in three WHO reference preparations. IgG 1 could be determined with satisfactory precision; for the other subclasses the quantification was less precise. The precision was dependent on the antisera for IgG 1, IgG 2 and IgG 4 but mainly on the calibrators for IgG 3. Mass units are to be preferred to relative units. WHO reference preparation 67 97 is proposed as a reference for determination of IgG subclasses in mass units. The target values are 5.0 g/l for IgG 1, 2.6 g/l for IgG 2, 0.4 g/l for IgG 3 and 0.5 g/l for IgG 4. These values add up to a total IgG value close to earlier WHO estimates.
