Two rice GRAS family genes responsive to N-acetylchitooligosaccharide elicitor are induced by phytoactive gibberellins:: evidence for cross-talk between elicitor and gibberellin signaling in rice cells
Two rice GRAS family genes responsive to N-acetylchitooligosaccharide elicitor are induced by phytoactive gibberellins:: evidence for cross-talk between elicitor and gibberellin signaling in rice cells
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DOI：
10.1023/b:plan.0000028792.72343.ee
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发表时间：
2004-01-01
影响因子：
5.1
通讯作者：
Minami, E
中科院分区：
文献类型：
--
作者：
Day, RB;Tanabe, S;Minami, E
在这项研究中，我们目前的数据显示，两个成员的GRAS家族的基因从水稻，CIGR 1和CIGR 2（几丁质诱导的赤霉素响应），诱导的有效激发子N-乙酰壳寡糖（GN），是快速诱导外源赤霉素。mRNA积累的模式依赖于赤霉素的剂量和生物活性，表明赤霉素对基因的诱导是由生物受体介导的，该生物受体能够在感知植物活性化合物时进行特异性识别和信号转导。进一步的药理学分析显示，通过用赤霉素处理的CIGR 1和CIGR 2 mRNA积累依赖于蛋白质磷酸化/去磷酸化事件。在来源于细长稻1（一种组成型赤霉素响应突变体，或d1，一种异源三聚体G蛋白α亚基缺陷的突变体）的水稻愈伤组织中，CIGR 1和CIGR 2被GN激发子诱导，而不是被赤霉素诱导。无论是赤霉素或GN表现出相关的活动，分别在防御或发展。这些结果表明，赤霉素的信号转导级联是独立的GN，并进一步暗示CIGR 1和CIGR 2在防御和发育中具有双重的，不同的作用。
In this study, we present data showing that two members of the GRAS family of genes from rice, CIGR1 and CIGR2 (chitin-inducible gibberellin-responsive), inducible by the potent elicitor N-acetylchitooligosaccharide (GN), are rapidly induced by exogenous gibberellins. The pattern of mRNA accumulation was dependent on the dose and biological activity of the gibberellins, suggesting that the induction of the genes by gibberellin is mediated by a biological receptor capable of specific recognition and signal transduction upon perception of the phytoactive compounds. Further pharmacological analysis revealed that the CIGR1 and CIGR2 mRNA accumulation by treatment with gibberellin is dependent upon protein phosphorylation/ dephosphorylation events. In rice calli derived from slender rice 1, a constitutive gibberellin- responsive mutant, or d1, a mutant deficient in the alpha-subunit of the heterotrimeric G-protein, CIGR1 and CIGR2 were induced by a GN elicitor, yet not by gibberellin. Neither gibberellin nor GN showed related activities in defense or development, respectively. These results strongly suggested that the signal transduction cascade from gibberellin is independent of that from GN, and further implied that CIGR1 and CIGR2 have dual, distinct roles in defense and development.