Shared features of blastula and neural crest stem cells evolved at the base of vertebrates.
Shared features of blastula and neural crest stem cells evolved at the base of vertebrates.
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囊胚和神经嵴干细胞的共同特征是在脊椎动物的基础上进化而来的。
DOI：
10.1101/2023.12.21.572714
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
LaBonne,Carole
中科院分区：
文献类型：
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作者：
York,JoshuaR;Rao,Anjali;Huber,PaulB;Schock,ElizabethN;Montequin,Andrew;Rigney,Sara;LaBonne,Carole
神经嵴是脊椎动物特有的干细胞群，有助于推动脊椎动物的起源和进化。这些细胞的一个显著特征是它们的多胚层潜能，这与另一种干细胞群脊椎动物囊胚的多能干细胞相似。在这里，我们调查神经嵴电位的进化起源，通过比较神经嵴和多能性基因调控网络的有颌脊椎动物，非洲爪蟾，和无颌脊椎动物，七鳃鳗。我们揭示了一个古老的进化起源共享的调控因子在这些基因调控网络，日期到现存的脊椎动物的最后一个共同祖先。专注于关键的多能性因子pou5，我们发现，七鳃鳗pou5的直系同源物在动物极细胞中表达，但没有从神经嵴。七鳃鳗和Xenopuspou 5都促进神经嵴的形成，这表明pou 5活性是从无颌脊椎动物的神经嵴中丢失的，或者是沿着沿着有颌脊椎动物的茎获得的。最后，我们提供的证据表明，pou5收购新的，神经嵴增强活动后，从祖先pou3样进化枝。这项工作提供的证据表明，神经嵴和囊胚多能性网络都出现在脊椎动物的基础上，这可能与pou5的功能进化有关。
The neural crest is a vertebrate-specific stem cell population that helped drive the origin and evolution of vertebrates. A distinguishing feature of these cells is their multi-germ layer potential, which has parallels to another stem cell population—pluripotent stem cells of the vertebrate blastula. Here, we investigate the evolutionary origins of neural crest potential by comparing neural crest and pluripotency gene regulatory networks of a jawed vertebrate,Xenopus, and a jawless vertebrate, lamprey. We reveal an ancient evolutionary origin of shared regulatory factors in these gene regulatory networks that dates to the last common ancestor of extant vertebrates. Focusing on the key pluripotency factor pou5, we show that a lampreypou5orthologue is expressed in animal pole cells but is absent from neural crest. Both lamprey andXenopuspou5 promote neural crest formation, suggesting that pou5 activity was lost from the neural crest of jawless vertebrates or acquired along the jawed vertebrate stem. Finally, we provide evidence that pou5 acquired novel, neural crest-enhancing activity after evolving from an ancestral pou3-like clade. This work provides evidence that both the neural crest and blastula pluripotency networks arose at the base of the vertebrates and that this may be linked to functional evolution of pou5.