Unequal functional redundancy between the two Arabidopsis thaliana high-affinity sulphate transporters SULTR1;1 and SULTR1;2
Unequal functional redundancy between the two Arabidopsis thaliana high-affinity sulphate transporters SULTR1;1 and SULTR1;2
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DOI：
10.1111/j.1469-8137.2008.02604.x
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发表时间：
2008-01-01
期刊：
影响因子：
9.4
通讯作者：
Gosti, Francoise
中科院分区：
文献类型：
--
作者：
Barberon, Marie;Berthomieu, Pierre;Gosti, Francoise
在拟南芥中，SULTR1;1 和 SULTR1;2 是两个被认为参与从土壤溶液中高亲和力吸收硫酸盐的基因。我们在此解决了它们在吸收硫酸盐和积累有毒类似物硒酸盐方面与植物生长和硒酸盐耐受性相关的功能冗余的具体问题。使用完整的基因型集，包括野生型、单个 sultr1;1 和 sultr1;2 突变体以及由此产生的双 sultr1;1-sultr1;2 突变体，我们对根长度进行了详细的表型分析，地上部生物量、硫酸盐吸收、硫酸盐和硒酸盐积累以及硒酸盐耐受性。结果均根据相同的功能层次对四种不同的基因型进行排序。野生型和 sultr1;1 突变体植物表现出相似的表型。相比之下，sultr1;1-sultr1;2双突变体植物表现出最极端的表型，而sultr1;2突变体表现出中等的表现。此外，根据所有基因型共有的单一 S 形回归曲线，硒酸耐受程度与幼苗的硒酸含量直接相关。SULTR1;1 和 SULTR1;2 基因显示出不等的功能冗余，这为 SULTR1;1 除了在硫酸盐膜转运中的作用外，还可能显示出额外的功能。
In Arabidopsis, SULTR1;1 and SULTR1;2 are two genes proposed to be involved in high-affinity sulphate uptake from the soil solution. We address here the specific issue of their functional redundancy for the uptake of sulphate and for the accumulation of its toxic analogue selenate with regard to plant growth and selenate tolerance.Using the complete set of genotypes, including the wild-type, each one of the single sultr1;1 and sultr1;2 mutants and the resulting double sultr1;1-sultr1;2 mutant, we performed a detailed phenotypic analysis of root length, shoot biomass, sulphate uptake, sulphate and selenate accumulation and selenate tolerance.The results all ordered the four different genotypes according to the same functional hierarchy. Wild-type and sultr1;1 mutant plants displayed similar phenotypes. By contrast, sultr1;1-sultr1;2 double-mutant plants showed the most extreme phenotype and the sultr1;2 mutant displayed intermediate performances. Additionally, the degree of selenate tolerance was directly related to the seedling selenate content according to a single sigmoid regression curve common to all the genotypes.The SULTR1;1 and SULTR1;2 genes display unequal functional redundancy, which leaves open for SULTR1;1 the possibility of displaying an additional function besides its role in sulphate membrane transport.