Quercitrin offers protection against brain injury in mice by inhibiting oxidative stress and inflammation
Quercitrin offers protection against brain injury in mice by inhibiting oxidative stress and inflammation
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DOI：
10.1039/c5fo00913h
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发表时间：
2016-01-01
期刊：
影响因子：
6.1
通讯作者：
Liu, Chan-Min
中科院分区：
文献类型：
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作者：
Ma, Jie-Qiong;Luo, Rong-Zhen;Liu, Chan-Min
槲皮苷是存在于蔬菜和水果中的主要类黄酮化合物之一。本研究的目的是评价槲皮苷对四氯化碳（CCl 4）诱导的脑损伤的影响，并进一步阐明其可能的机制。ICR小鼠腹腔内接受CCl 4与或不与槲皮苷联合给药4周。结果表明，槲皮苷能显著抑制小鼠脑组织中活性氧（ROS）的产生和丙二醛（MDA）含量的升高，降低组织型纤溶酶原激活物（t-PA）活性，增强抗氧化酶活性，消除细胞色素P450 2 E1（CYP 2 E1）的诱导作用。槲皮苷还可预防CCl 4诱导的脑功能障碍，这与其抑制单胺氧化酶（MAO）、乙酰胆碱酯酶（AChE）和N-甲基-D-天冬氨酸受体2B亚单位（NR 2B）的活性有关。此外，蛋白质印迹分析表明，槲皮苷抑制促炎细胞因子如肿瘤坏死因子α（TNF-α）和白细胞介素-6（IL-6）的释放。综上所述，我们的研究结果表明，槲皮苷可能是一个潜在的候选人被开发为一种神经保护剂。
Quercitrin is one of the primary flavonoid compounds present in vegetables and fruits. The aim of the present study was to evaluate the effects of quercitrin against carbon tetrachloride (CCl4) induced brain injury and further to elucidate its probable mechanisms. ICR mice received CCl4 intraperitoneally with or without quercitrin co-administration for 4 weeks. Our data showed that quercitrin significantly suppressed the elevation of reactive oxygen species (ROS) production and malondialdehyde (MDA) content, reduced tissue plasminogen activator (t-PA) activity, enhanced the antioxidant enzyme activities and abrogated cytochrome P450 2E1 (CYP2E1) induction in mouse brains. Quercitrin also prevented CCl4 induced cerebral function disorders associated with its ability to inhibit the activities of monoamine oxidase (MAO), acetylcholine esterase (AChE) and the N-methyl-D-aspartate receptor 2B subunit (NR2B). In addition, western blot analysis showed that quercitrin suppressed the release of pro-inflammatory cytokines such as tumor necrosis factor alpha (TNF-alpha) and interleukin-6 (IL-6). Taken together, our findings suggested that quercitrin may be a potential candidate to be developed as a neuroprotective agent.