Evaluation of culture conditions for cellulase production by two Trichoderma reesei mutants under solid-state fermentation conditions
Evaluation of culture conditions for cellulase production by two Trichoderma reesei mutants under solid-state fermentation conditions
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DOI：
10.1016/j.biortech.2006.11.019
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发表时间：
2007-12-01
影响因子：
11.4
通讯作者：
Barzegar, Mohsen
中科院分区：
文献类型：
--
作者：
Latifian, Maryam;Hamidi-Esfahani, Zohreh;Barzegar, Mohsen
采用响应面法（RSM）评价发酵参数对里氏木霉QM9414和里氏木霉MCG77在以米糠为底物的固态发酵中生产纤维素酶的影响。使用滤纸活性 (FPA) 作为响应来优化初始 pH、水分含量和温度。对里氏木霉QM9414结果的统计分析表明，只有水分含量对纤维素酶活性有显着影响，并且对酶活性具有线性影响（在70%水分含量时获得最大酶活性）。里氏木星 MCG77 的结果表明，温度和水分含量是纤维素酶活性最重要的参数。纤维素酶的最佳生产温度为25-30℃，水分含量为55%至70%。优化后，里氏木霉MCG77的FPA比里氏木霉QM9414提高了2.5倍。 (C) 2006 Elsevier Ltd. 保留所有权利。
Response surface methodology (RSM) was used to evaluate the effects of fermentation parameters for cellulase production by Trichoderma reesei QM9414 and T reesei MCG77 in solid-state fermentation using rice bran as substrate. Initial pH, moisture content and temperature were optimized using filter paper activity (FPA) as response. Statistical analysis of the results for T reesei QM9414 showed that only moisture content had significant effect on cellulase activity and had a linear effect on enzyme activity (maximum enzyme activities were obtained at 70% moisture content). The results for T reesei MCG77 showed that temperature and moisture content were the most significant parameters for cellulase activity. The optimum cellulase production was in the temperature range of 25-30 degrees C and moisture content between 55% and 70%. After the optimization, the FPA in T reesei MCG77 was increased by 2.5 folds compared to that of T. reesei QM9414. (C) 2006 Elsevier Ltd. All rights reserved.