Triticumoside induces apoptosis via caspase-dependent mitochondrial pathway and inhibits migration through downregulation of MMP2/9 in human lung cancer cells
Triticumoside induces apoptosis via caspase-dependent mitochondrial pathway and inhibits migration through downregulation of MMP2/9 in human lung cancer cells
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DOI：
10.1093/abbs/gmv124
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发表时间：
2016-02-01
影响因子：
3.7
通讯作者：
Kim, Dae-Ki
中科院分区：
文献类型：
--
作者：
Poudel, Barun;Ki, Hyeon-Hui;Kim, Dae-Ki
非小细胞肺癌（NSCLC）是全球主要的癌症相关死亡，其5年生存率仅为14%。小麦皂苷是小麦芽提取物中的一种酚类化合物，具有抗肥胖和抗炎作用。然而，麦草皂苷对癌细胞增殖和迁移的影响尚未得到研究。为了阐明麦草苷是否具有抗癌作用，我们用不同剂量的麦草苷培养细胞，通过观察细胞活力、细胞形态变化和膜联蛋白- v -异硫氰酸荧光素/碘化丙啶染色来评估细胞凋亡。细胞周期分析、免疫印迹、伤口愈合试验和定量聚合酶链反应也被执行。麦草皂苷对细胞具有明显的细胞毒性，且呈剂量和时间依赖性。麦草皂苷引起细胞变圆、核凝聚和收缩等形态学改变。同样，麦草苷也能提高细胞的亚g1比例。此外，麦草苷调节凋亡相关蛋白的表达，如b细胞淋巴瘤2 （Bcl-2）、Bcl-2相关的X和procaspase-3/9。麦草皂苷还通过下调基质金属蛋白酶2/9 （MMP2/9）抑制细胞迁移。综上所述，这些结果表明，在NSCLC A549细胞中，麦草苷通过caspase依赖的线粒体途径诱导细胞凋亡，并通过抑制MMP2/9来抑制迁移。
Non-small cell lung cancer (NSCLC) is the major cancer-related death worldwide with only 14% five-year survival rate. Triticumoside, a phenolic compound present in Triticum aestivum sprout extract, has been recognized to have antiobesity and anti-inflammatory effects. However, the effect of triticumoside on cancer cell proliferation and migration has not been studied. In order to elucidate whether triticumoside exhibits an anticancer effect, cells were incubated with different doses of triticumoside, and apoptosis was assessed by observing cell viability, cellular morphological changes, and annexin-V-fluorescein isothiocyanate/propidium iodide staining. Cell cycle analysis, western blotting, wound healing assay, and quantitative-polymerase chain reaction were also performed. Triticumoside exhibited marked cytotoxicity in the cells in dose-and time-dependent manner. Triticumoside caused morphological changes, including cellular rounding, nuclear condensation, and shrinkage. Likewise, triticumoside enhanced the sub-G1 proportion of cells. Additionally, triticumoside regulated expression of apoptosis-associated proteins, such as B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X, and procaspase-3/9. Triticumoside also inhibited migration of the cells through downregulation of matrix metalloproteinase-2/9 (MMP2/9). Collectively, these results suggest that triticumoside induces apoptosis through caspase-dependent mitochondrial pathway and suppresses migration via inhibition of MMP2/9 in NSCLC A549 cells.