Biodegradation of skatole by Burkholderia sp. IDO3 and its successful bioaugmentation in activated sludge systems
Biodegradation of skatole by Burkholderia sp. IDO3 and its successful bioaugmentation in activated sludge systems
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伯克霍尔德氏菌对粪臭素的生物降解。
DOI：
10.1016/j.envres.2020.109123
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发表时间：
2020
影响因子：
8.3
通讯作者：
Sun Yeqing
中科院分区：
文献类型：
--
作者：
Ma Qiao;Qu Hui;Meng Nan;Li Shuzhen;Wang Jingwei;Liu Shengwei;Qu Yuanyuan;Sun Yeqing
粪臭素是畜禽粪便和废水中的主要恶臭化合物，具有较低的气味阈值。它不仅对居民和工人造成严重的危害，而且由于其生物毒性和顽固性，对环境和人类健康构成威胁。生物处理是一种生态友好、经济有效的粪垢去除方法，但细菌资源稀缺。本文首次报道了burkholderastrain对粪臭素的有效降解。结果表明，菌株IDO3在pH 4.0 ~ 9.0、转速0 ~ 250 rpm、温度30 ~ 35℃条件下均能保持较高的降解滑石性能。RNA-seq分析表明，粪臭素激活了菌株IDO3的氧化磷酸化和ATP生成水平。基因本体分析对373个不同表达基因的氧化还原酶活性项影响显著。此外，生物强化实验表明，菌株IDO3可以显著提高活性污泥系统中臭臭素的去除率。高通量16S rRNA基因测序数据显示，术后8 d生物增强组α -多样性和细菌群落趋于稳定。PICRUSt分析显示，外源性生物降解和代谢以及膜运输类别显著增加，与生物增强组的粪臭素去除性能改善一致。在整个操作过程中，burkholderia存活并定植为优势种群（34.19-64.00%），证实了burkholderia的可行性。IDO3在复杂体系中的生物增强作用。
Skatole is the key malodorous compound in livestock and poultry waste and wastewater with a low odor threshold. It not only causes serious nuisance to residents and workers, but also poses threat to the environment and human health due to its biotoxicity and recalcitrant nature. Biological treatment is an eco-friendly and cost-effective approach for skatole removal, while the bacterial resources are scarce. Herein, theBurkholderiastrain was reported to efficiently degrade skatole for the first time. Results showed that strain IDO3 maintained high skatole-degrading performance under the conditions of pH 4.0–9.0, rotate speed 0–250 rpm, and temperature 30–35 °C. RNA-seq analysis indicated that skatole activated the oxidative phosphorylation and ATP production levels in strain IDO3. The oxidoreductase activity item which contained 373 differently expressed genes was significantly impacted by Gene Ontology analysis. Furthermore, the bioaugmentation experiment demonstrated that strain IDO3 could notably increase the removal of skatole in activated sludge systems. High-throughput 16S rRNA gene sequencing data indicated that the alpha-diversity and bacterial community tended to be stable in the bioaugmented group after 8 days operation. PICRUSt analysis indicated that xenobiotics biodegradation and metabolism, and membrane transport categories significantly increased, consistent with the improved skatole removal performance in the bioaugmented group.Burkholderiawas survived and colonized to be the predominant population during the whole operation process (34.19–64.00%), confirming the feasibility ofBurkholderiasp. IDO3 as the bioaugmentation agent in complex systems.