Cartilage engineering using cell-derived extracellular matrix scaffold in vitro
Cartilage engineering using cell-derived extracellular matrix scaffold in vitro
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DOI：
10.1002/jbm.a.32419
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发表时间：
2010-03-15
影响因子：
4.9
通讯作者：
Min, Byoung-Hyun
中科院分区：
文献类型：
--
作者：
Jin, Cheng Zhe;Choi, Byung Hyune;Min, Byoung-Hyun
以猪软骨细胞为材料，采用冷冻干燥法构建细胞源性细胞外基质（ECM）支架，并评价其体外促进软骨形成的能力。扫描电子显微镜（SEM）显示支架具有高度均匀的多孔结构。将兔软骨细胞动态接种于ECM支架上，体外培养2 d、1、2、4周后进行分析。聚乙醇酸（PGA）支架用作对照。大体观察新生软骨组织，在ECM支架中培养1周后观察到银白色白色软骨样组织，而在PGA支架中仅在4周后观察到类似的形态。ECM组和PGA组的新生软骨样组织体积均显著增加。ECM组的抗压强度随时间的延长而逐渐增加，PGA组的抗压强度随时间的延长而逐渐降低。DNA、糖胺聚糖（GAG）和胶原含量在两组中也随时间逐渐增加，但ECM组增加更明显。GAG（番红0染色）和11型胶原（免疫组织化学）的组织学染色显示ECM分子随时间持续积累，其逐渐且均匀地填充ECM支架中的多孔空间。相反，它们仅聚集在PGA支架的周边区域。这些结果表明，一种新的细胞来源的ECM支架可以提供一个有前途的环境，在体外产生高质量的软骨。(C)2009 Wiley Periodicals，Inc. J Biomed Mater Res 92A：1567-1577，2010
A cell-derived extracellular matrix (ECM) scaffold was constructed using cultured porcine chondrocytes via a freeze-drying method, and its ability to promote cartilage formation was evaluated in vitro. Scanning electron microscope (SEM) revealed that the scaffold had highly uniform porous microstructure. Then, rabbit chondrocytes were seeded dynamically on ECM scaffold and cultured for 2 days, 1, 2, and 4 weeks in vitro for analysis. Polyglycolic acid (PGA) scaffold was used as a control. On gross observation of neocartilage tissue, a silvery white cartilage-like tissue was observed after I week of culture in ECM scaffold, while similar morphology was seen only after 4 weeks in PGA scaffold. The volume of neocartilage-like tissue was significantly increased in both ECM and PGA groups. The compressive strength was gradually increased with time in ECM group, while gradually decreased in PGA group. DNA, glycosaminoglycan (GAG) and collagen contents also increased gradually with time in both groups, but showed more significant increase in ECM group. Histological staining for GAG (Safranin 0 staining) and type 11 collagen (immunohistochemistry) showed sustained accumulation of ECM molecules with time, which gradually and uniformly filled porous space in ECM scaffold. On the contrary, they accumulated only at the peripheral area of PGA scaffold. These results suggest that a novel cell-derived ECM scaffold can provide a promising environment for generating a high quality cartilage in vitro. (C) 2009 Wiley Periodicals, Inc. J Biomed Mater Res 92A: 1567-1577, 2010