Diurnal variations of tumor necrosis factor alpha mRNA and alpha-tubulin mRNA in rat brain
Diurnal variations of tumor necrosis factor alpha mRNA and alpha-tubulin mRNA in rat brain
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DOI：
10.1159/000097325
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发表时间：
1997-03-01
影响因子：
2.4
通讯作者：
Krueger, JM
中科院分区：
文献类型：
--
作者：
Bredow, S;GuhaThakurta, N;Krueger, JM
本文所述的实验旨在确定肿瘤坏死因子α（TNF-α）是否在正常大鼠脑的各个区域中表现出昼夜变化。通过逆转录酶聚合酶链反应检测TNF-α mRNA转录物。为了监测TNF-α和α-微管蛋白表达的昼夜变化，从光照开始后1小时开始，每4小时处死大鼠一次，持续24小时；测定小脑、皮质、海马、下丘脑和脑干的相对mRNA水平。下丘脑和海马的 TNF-α mRNA 在光相期间高于暗相，α-微管蛋白 mRNA 在下丘脑、海马和皮层中表现出类似的昼夜变化，相反，海马和皮层的 β-肌动蛋白 mRNA 在光相期间低于暗相。观察到的 TNF-α mRNA 的昼夜变化与 TNF 在大脑中具有生理作用的假设一致。
The experiments described herein were designed to determine whether tumor necrosis factor alpha (TNF-alpha) displays a diurnal variation in various areas of the normal rat brain. TNF-alpha mRNA transcripts were detected by reverse-transcriptase polymerase chain reaction. To monitor diurnal changes in TNF-alpha and alpha-tubulin expression, rats were sacrificed every 4 h for 24 h starting I h after light onset; relative mRNA levels were determined fur the cerebellum, cortex, hippocampus, hypothalamus and brainstem. TNF-alpha mRNA was higher during the light than in the dark phase in the hypothalamus and hippocampus, alpha-Tubulin mRNA exhibited a similar diurnal variation in the hypothalamus, hippocampus and cortex, In contrast, beta-actin mRNA was lower during the light phase than the dark phase in the hippocampus and cortex. The observed diurnal variations in TNF-alpha mRNA are consistent with the hypothesis that TNF has a physiological role in the brain.