Analysis of tissue flow patterns during primitive streak formation in the chick embryo
Analysis of tissue flow patterns during primitive streak formation in the chick embryo
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DOI：
10.1016/j.ydbio.2005.04.021
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发表时间：
2005-08-01
影响因子：
2.7
通讯作者：
Weijer, CJ
中科院分区：
文献类型：
--
作者：
Cui, C;Yang, XS;Weijer, CJ
我们研究了鸡胚原条形成的组织流动模式。分析的时间推移序列的明场图像提取的组织速度场和荧光图像的小团体的二碘标记的细胞表明，外胚层细胞移动在两个大规模的反向旋转流，合并在网站的条纹形成。尽管有大规模的组织流动，但单个细胞似乎相对于它们的邻居几乎没有移动。当条纹形成时，它在前后两个方向上都伸长。通过局部应用抑制剂latrunculin A抑制肌动蛋白聚合，立即终止条纹尖端的前延伸，但不能阻止后延伸。抑制肌动蛋白聚合的基础上的条纹完全抑制条纹的形成，这意味着细胞的连续运动到基地的形成条纹是至关重要的延伸。早期胚胎细胞周期的分析表明，细胞周期的进展是相当均匀的原始条纹形成之前，在中央外胚层和初始条纹减少和增加之间的边界透明区和不透明区在伸长。细胞周期抑制剂aphidicolin在完全阻断细胞周期进程的浓度下，允许初始条纹形成，但在延伸过程中阻止发育。我们的分析表明，细胞分裂维持细胞流动模式，提供条纹与细胞从外侧上胚层，这是至关重要的上胚层扩张在周边地区，但分裂不驱动条纹形成或观察到的组织流。(c)2005年由Elsevier Inc.出版
We have investigated the patterns of tissue flow underlying the formation of the primitive streak in the chick embryo. Analysis of time-lapse sequences of brightfield images to extract the tissue velocity field and of fluorescence images of small groups of DiI-labelled cells have shown that epiblast cells move in two large-scale counter-rotating streams, which merge at the site of streak formation. Despite the large-scale tissue flows, individual cells appear to move little relative to their neighbours. As the streak forms, it elongates in both the anterior and posterior directions. Inhibition of actin polymerisation via local application of the inhibitor latrunculin A immediately terminates anterior extension of the streak tip, but does not prevent posterior elongation. Inhibition of actin polymerisation at the base of the streak completely inhibits streak formation, implying that continuous movement of cells into the base of the forming streak is crucial for extension. Analysis of cycling cells in the early embryo shows that cell-cycle progression in the epiblast is quite uniform before the primitive streak forms then decreases in the central epiblast and incipient streak and increases at the boundary between the area pellucida and area opaca during elongation. The cell-cycle inhibitor aphidicolin, at concentrations that completely block cell-cycle progression, permits initial streak formation but arrests development during extension. Our analysis suggests that cell division maintains the cell-flow pattern that supplies the streak with cells from the lateral epiblast, which is critical for epiblast expansion in peripheral areas, but that division does not drive streak formation or the observed tissue flow. (c) 2005 Published by Elsevier Inc.