Racial differences in androgen receptor protein expression in men with clinically localized prostate cancer
Racial differences in androgen receptor protein expression in men with clinically localized prostate cancer
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DOI：
10.1097/01.ju.0000079761.56154.e5
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发表时间：
2003-09-01
影响因子：
6.6
通讯作者：
Mohler, JL
中科院分区：
文献类型：
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作者：
Gaston, KE;Kim, D;Mohler, JL
目的：与白色美国男性相比，美国黑人男性前列腺癌（CaP）的死亡率不成比例。结果的差异可能源于雄激素轴内的差异。由于血清睾酮水平似乎是相似的，在男性与CaP的种族，我们测量和比较雄激素受体（AR）蛋白表达的恶性和良性前列腺组织从黑色和白色男子进行根治性前列腺切除术的临床本地化CaP.Materials和方法：存档根治性前列腺切除术标本从25白色和25名黑人男性AR蛋白抗原检索和免疫染色。用2种方法检测CaP和良性组织中AR蛋白的表达。使用自动数字彩色视频图像分析来测量AR蛋白免疫染色的面积百分比和表达强度（平均光密度）。视觉评分进行比较结果与automaticvalue.Results：在黑色相比，白色男子恶性细胞核27%更可能免疫染色AR（P = 0.005）和免疫阳性细胞核AR蛋白表达是81%以上（P = 0.002）。恶性细胞核的视觉评分显示，AR免疫染色在黑人与白色男性中显著增加（171 +/- 40 vs 149 +/- 37，p = 0.048）。在免疫阳性良性核AR蛋白表达是22%，黑人比白色男性（p = 0.027）。良性细胞核的视觉评分显示，黑人与白色男性相比，免疫染色增加了20%，尽管这种差异没有达到统计学显著性（p = 0.065）。尽管黑人男性的血清前列腺特异性抗原水平较高，但AR蛋白表达的种族差异无法用年龄、病理分级或分期来解释（9.7 +/- 7.5 vs 15.5 +/- 12.2 ng/ml，p = 0.049）。与白色男性相比，非洲黑人男性的良性前列腺AR蛋白表达高22%，CaP高81%。由于前列腺的雄激素刺激的种族差异，CaP可能发生在较年轻的年龄，并且黑人比白色男性进展更快。
Purpose: Black American men experience disproportionate mortality from prostate cancer (CaP) compared with white American men. Differences in outcome may stem from differences within the androgen axis. Since serum testosterone levels appear to be similar by race in men with CaP, we measured and compared androgen receptor (AR) protein expression in malignant and benign prostate tissue from black and white men who underwent radical prostatectomy for clinically localized CaP.Materials and Methods: Archived radical prostatectomy specimens obtained from 25 white and 25 black men had AR protein antigen retrieved and immunostained. AR protein expression from CaP and benign tissue was assessed by 2 methods. Automated digital color video image analysis was used to measure the percent area immunostained for AR protein and the intensity of expression (mean optical density). Visual scoring was performed to compare results with automated values.Results: In black compared with white men malignant nuclei were 27% more likely to immunostain for AR (p = 0.005) and in immunopositive nuclei AR protein expression was 81% greater (P = 0.002). Visual scoring of malignant nuclei revealed that AR immunostaining was significantly increased in black vs white men (171 +/- 40 vs 149 +/- 37, p = 0.048). In immunopositive benign nuclei AR protein expression was 22% greater in black than in white men (p = 0.027). Visual scoring of benign nuclei revealed 20% increased immunostaining in black vs white men, although this difference did not attain statistical significance (p = 0.065). Racial differences in AR protein expression were not explained by age, pathological grade or stage, although serum prostate specific antigen levels were higher in black men (9.7 +/- 7.5 vs 15.5 +/- 12.2 ng/ml, p = 0.049).Conclusions: AR protein expression was 22% higher in the benign prostate and 81% higher in the CaP of black African compared with white men. CaP may occur at a younger age and progress more rapidly in black than in white men due to racial differences in androgenic stimulation of the prostate.