Seedling Chloroplast Responses Induced by N -Linolenoylethanolamine Require Intact G-Protein Complexes
Seedling Chloroplast Responses Induced by N -Linolenoylethanolamine Require Intact G-Protein Complexes
复制标题
N-亚麻酰乙醇胺诱导的幼苗叶绿体反应需要完整的 G 蛋白复合物
DOI：
10.1104/pp.19.01552
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发表时间：
2020
期刊：
影响因子：
7.4
通讯作者：
Chapman, Kent D.
中科院分区：
文献类型：
--
作者：
Yan, Chengshi;Cannon, Ashley E.;Watkins, Justin;Keereetaweep, Jantana;Khan, Bibi Rafeiza;Jones, Alan M.;Blancaflor, Elison B.;Azad, Rajeev K.;Chapman, Kent D.
在动物体内，一些长链乙醇胺(NAE)已被鉴定为内源性大麻素，它们是通过细胞表面G蛋白偶联的大麻素受体发挥作用的自分泌信号。尽管NAE存在于包括非维管束植物在内的陆地植物中，但其确切的信号特性和分子靶标还没有很好的确定。在这里，我们表明，N-亚麻酰乙醇胺(NAE 18：3)的活性需要一个完整的G-蛋白复合体。具体地说，Gβγ二聚体的遗传消融或全套非典型Gα亚基的丢失强烈地减弱了NAE-18：3诱导的拟南芥子叶脱色。这种效应至少部分地涉及到对叶绿素生物合成和分解代谢基因的转录调控。此外，还有G-蛋白信号成分和G-蛋白相互作用因子的前馈转录控制。这些结果与NAE 18：3是植物中需要G蛋白介导信号转导的脂质信号分子是一致的，这种情况与NAE内源性大麻素在动物系统中的作用相似，但不完全相同。
In animals, several long-chainN-acylethanolamines (NAEs) have been identified as endocannabinoids and are autocrine signals that operate through cell surface G-protein–coupled cannabinoid receptors. Despite the occurrence of NAEs in land plants, including nonvascular plants, their precise signaling properties and molecular targets are not well defined. Here we show that the activity ofN-linolenoylethanolamine (NAE 18:3) requires an intact G-protein complex. Specifically, genetic ablation of the Gβγ dimer or loss of the full set of atypical Gα subunits strongly attenuates an NAE-18:3–induced degreening of cotyledons in Arabidopsis (Arabidopsis thaliana) seedlings. This effect involves, at least in part, transcriptional regulation of chlorophyll biosynthesis and catabolism genes. In addition, there is feedforward transcriptional control of G-protein signaling components and G-protein interactors. These results are consistent with NAE 18:3 being a lipid signaling molecule in plants with a requirement for G-proteins to mediate signal transduction, a situation similar, but not identical, to the action of NAE endocannabinoids in animal systems.