Identification and characterization of a novel phosphorylation site on the GluR1 subunit of AMPA receptors
Identification and characterization of a novel phosphorylation site on the GluR1 subunit of AMPA receptors
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DOI：
10.1016/j.mcn.2007.06.003
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发表时间：
2007-09-01
影响因子：
3.5
通讯作者：
Huganir, Richard L.
中科院分区：
文献类型：
--
作者：
Lee, Hey-Kyoung;Takamiya, Kogo;Huganir, Richard L.
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Phosphorylation of various AMPA receptor subunits can alter synaptic transmission and plasticity at excitatory glutamatergic synapses in the central nervous system. Here, we identified threonine-840 (T840) on the GluR1 subunit of AMPA receptors as a novel phosphorylation site. T840 is phosphorylated by protein kinase C (PKC) in vitro and is a highly turned-over phosphorylation site in the hippocampus. Interestingly, the high basal phosphorylation of T840 in the hippocampus is maintained by a persistent activity of a protein kinase, which is connter-balanced by a basal protein phosphatase activity. To study the function of T840, we generated a line of mutant mice lacking this phosphorylation site using a gene knock-in technique. The mice generated lack T840, in addition to two previously identified phosphorylation sites S831 and S845. Using this mouse, we demonstrate that T840 may regulate synaptic plasticity in an age-dependent manner. (c) 2007 Elsevier Inc. All rights reserved.